Flavobacterium Psychrophilum antibody | FL43
While F. pyschrophilum infections are primarily in young fish, with Rainbow trout and Coho salmon being particularly susceptable, salmonids of all ages are subject to infection. Additionally F. pyschrophilum has been detected in a range of non salmonids including species from eel, (Anguilla sp.), and carp (Carassius sp.) families (Barnes et al. 2011).
While the route of infection is not fully understood, it is beleived that infection may in part be from parent to offspring, however it is known that F. psychrophilum survives outside its host in an aquatic environment for a considerable time period. Additionally other fish, both inter- and intraspecies can function as a reservoir for infection, particulary dying and dead individuals that have been demonstrated to shed increased levels of bacteria (Madetoja et al. 2000). Infection results in a range of clinical symptoms including necrosis of tissues in such as the fins, enlarged organs, in particular, the spleen, neurological damage and morphological deformities. Mortality varies widely from 10% to over 75% and it is believed that the variation in mortality may be affected by a combination of factors including, but not limited to, bacterial strain virulence, water temperature, genetic diversity within fish stocks and stress (Barnes et al. 2011).
Clone FL43 has been shown to be specific for F.psychrophilum and does not recognise strains of the closely related F. columnare, F. pectinovorum, F. aquatile, F. branchiophilum, and F. saccharophilumte tested to date. Clone FL43 may therefore be used to identify F. psycophilum in tissues of infected individuals and may be of use in research to develop treatment regimes for farmed salmonid species (Lindstrom et al. 2009) .
- Target Species
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
- Outer membrane fractions of F. psychrophilum (wildtype strain CSF 259-95)
- Approx. Protein Concentrations
- IgG concentration 0.73 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the Mouse X63-Ag22.214.171.124 myeloma cell line.
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
- 18 months from date of despatch.
- For research purposes only
Applications of Flavobacterium Psychrophilum antibody
|Application Name||Verified||Min Dilution||Max Dilution|
Useful Reagents Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Rabbit anti Salmonid Ig||AHP761||E IF WB||50 µl|
|Mouse anti Salmonid Ig||MCA2182||E F IF WB||0.5 ml|
|Mouse anti Rainbow Trout Ig||MCA5976||C E IF WB||0.5 ml|
Product Specific References
References for Flavobacterium Psychrophilum antibody
Lindstrom, N. M, et al. (2009) A quantitative enzyme-linked immunosorbent assay and filtration-based fluorescent antibody test as potential tools to screen broodstock for infection with Flavobacterium psychrophilum.
J Aquat Anim Health. 21: 43-56.
Gliniewicz, K. et al. (2012) Comparative proteomic analysis of virulent and rifampicin-attenuated Flavobacterium psychrophilum.
J Fish Dis. 35 (7): 529-39.
Madetoja, J., et al. (2000) Flavobacterium psychrophilum, invasion into and shedding by rainbow trout Oncorhynchus mykiss.
Int J Parasitol. 30: 321-6.
Barnes, M.E. et al. (2011) A Review of Flavobacterium Psychrophilum Biology, Clinical Signs, and Bacterial Cold Water Disease Prevention and Treatment.
The Open Fish Science Journal 4: 40-8.