Flavobacterium Psychrophilum antibody | FL43

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Mouse anti Flavobacterium Psychrophilum:FITC

Product Type
Monoclonal Antibody
Clone
FL43
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5978F IF 0.1 mg
Mouse anti Flavobacterium psychrophilum, clone FL43, recognizes the infectious bacteria Flavobacterium psychrophilum, previously designated as Cytophaga psychrophila and Flexibacter psychrophilus. F. pschrophillum is the causative agent of fish disease known under a range names, most commonly as Bacterial cold water disease (BCWD) in America and Rainbow trout fry syndrome (RTFS) in Europe. F. psychrophilum has a worldwide distribution where it is responsible for serious disease outbreaks in predominantly freshwater salmonid fish farms and hatcheries resulting in significant ecconomic loss for the aquaculture industry.

While F. pyschrophilum infections are primarily in young fish, with Rainbow trout and Coho salmon being particularly susceptable, salmonids of all ages are subject to infection. Additionally F. pyschrophilum has been detected in a range of non salmonids including species from eel, (Anguilla sp.), and carp (Carassius sp.) families (Barnes et al. 2011).

While the route of infection is not fully understood, it is beleived that infection may in part be from parent to offspring, however it is known that F. psychrophilum survives outside its host in an aquatic environment for a considerable time period. Additionally other fish, both inter- and intraspecies can function as a reservoir for infection, particulary dying and dead individuals that have been demonstrated to shed increased levels of bacteria (Madetoja et al. 2000). Infection results in a range of clinical symptoms including necrosis of tissues in such as the fins, enlarged organs, in particular, the spleen, neurological damage and morphological deformities. Mortality varies widely from 10% to over 75% and it is believed that the variation in mortality may be affected by a combination of factors including, but not limited to, bacterial strain virulence, water temperature, genetic diversity within fish stocks and stress (Barnes et al. 2011).

Clone FL43 has been shown to be specific for F.psychrophilum and does not recognise strains of the closely related F. columnare, F. pectinovorum, F. aquatile, F. branchiophilum, and F. saccharophilumte tested to date. Clone FL43 may therefore be used to identify F. psycophilum in tissues of infected individuals and may be of use in research to develop treatment regimes for farmed salmonid species (Lindstrom et al. 2009) .

Product Details

Target Species
Bacterial
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Outer membrane fractions of F. psychrophilum (wildtype strain CSF 259-95)
Approx. Protein Concentrations
IgG concentration 0.73 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the Mouse X63-Ag8.6.5.3 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Flavobacterium Psychrophilum antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/100 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Salmonid Ig AHP761 50 µl E IF WB
Mouse anti Salmonid Ig MCA2182 0.5 ml E F IF WB
Mouse anti Rainbow Trout Ig MCA5976 0.5 ml C E IF WB

Product Specific References

References for Flavobacterium Psychrophilum antibody

  1. Lindstrom, N. M, et al. (2009) A quantitative enzyme-linked immunosorbent assay and filtration-based fluorescent antibody test as potential tools to screen broodstock for infection with Flavobacterium psychrophilum.
    J Aquat Anim Health. 21: 43-56.
  2. Gliniewicz, K. et al. (2012) Comparative proteomic analysis of virulent and rifampicin-attenuated Flavobacterium psychrophilum.
    J Fish Dis. 35 (7): 529-39.

Further Reading

  1. Madetoja, J., et al. (2000) Flavobacterium psychrophilum, invasion into and shedding by rainbow trout Oncorhynchus mykiss.
    Int J Parasitol. 30: 321-6.
  2. Barnes, M.E. et al. (2011) A Review of Flavobacterium Psychrophilum Biology, Clinical Signs, and Bacterial Cold Water Disease Prevention and Treatment.
    The Open Fish Science Journal 4: 40-8.