Clostridium tetani Tetanus Toxin antibody | TetH6
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Mouse anti Clostridium tetani Tetanus Toxin antibody, clone TetH6 recognizes the tetanus toxin, a neurotoxin produced by the vegetative spore of Clostridium tetani. The toxin causes tetanus, a disease that is characterised by prolonged contraction of skeletal muscle. World-wide, approximately 1 million cases of tetanus occurs annually, resulting in approximately 300,000 to 500,000 deaths, mostly in developing countries. The tetanus toxin has a molecular weight of approximately 150 kDa and consists of a heavy chain (B-chain, 100 kDa) and a light chain (A-chain, 50 kDa) that are connected by a disulfide bond. The toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits the release of the neurotransmitters GABA and glycine by synaptobrevin-2. This results in overactivity in the muscle. The binding of the toxin to neurons is irreversible.
Mouse anti Clostridium tetani Tetanus Toxin antibody, clone TetH6 has toxin neutralising activity.
Mouse anti Clostridium tetani Tetanus Toxin antibody, clone TetH6 has toxin neutralising activity.
Product Details
- Target Species
- Bacterial
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Supernatant from Clostridium tetani.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P04958 Related reagents
- GO Terms
- GO:0004222 metalloendopeptidase activity
- GO:0005576 extracellular region
- GO:0006508 proteolysis
- GO:0008270 zinc ion binding
- GO:0009405 pathogenesis
- GO:0050827 toxin receptor binding
- GO:0051609 inhibition of neurotransmitter uptake
- Regulatory
- For research purposes only
Applications of Clostridium tetani Tetanus Toxin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Functional Assays | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Clostridium tetani Tetanus Toxin antibody
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Pan, S. et al. (2012) The study on newly developed McAb NJ001 specific to non-small cell lung cancer and its biological characteristics.
PLoS One. 7 (3): e33009.
