Clostridium difficile Toxin B antibody | 5156
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Mouse anti Clostridium difficile Toxin B antibody, clone 5156 recognizes Clostridium difficile toxin B. No reaction is observed with toxin A. Clostridium difficile is a gram-positive motile bacterium which is the leading cause of diarrhoea in developed countries. The incidence of disease in humans varies greatly with age, spore density and the administration of antibiotics.
Toxin B (TcdB) is a large exotoxin. Its role in disease is less well understood than that of toxin A, though it seems clear that TcdB contributes to disease. Both toxins modify the Ras superfamily of small GTPases via glycosylation, inactivating them and leading to the disruption of vital signaling pathways in the cell.
Toxin B (TcdB) is a large exotoxin. Its role in disease is less well understood than that of toxin A, though it seems clear that TcdB contributes to disease. Both toxins modify the Ras superfamily of small GTPases via glycosylation, inactivating them and leading to the disruption of vital signaling pathways in the cell.
Product Details
- Target Species
- Bacterial
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P18177
- GO Terms
- GO:0009405 pathogenesis
- GO:0046527 glucosyltransferase activity
- Regulatory
- For research purposes only
Applications of Clostridium difficile Toxin B antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/20 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
Further Reading
-
Voth, D.E. & Ballard, J.D. (2005) Clostridium difficile toxins: mechanism of action and role in disease.
Clin Microbiol Rev. 18 (2): 247-63.
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