Clostridium difficile Toxin A antibody | 5155
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Mouse anti Clostridium dificile toxin A antibody, clone 5155 recognizes Clostridium difficile toxin A. No reaction is observed with Clostridium difficile toxin B, tetanus toxoid, Pseudomonas aeruginosa and Yersinia enterocolitica
Clostridium difficile is a gram-positive motile bacterium which is the leading cause of diarrhoea in developed countries. The incidence of disease in humans varies greatly with age, spore density and the administration of antibiotics.
Toxin A (TcdA) is a large exotoxin which is an essential virulence factor for C. difficile infection. TcdA has a molecular mass of ~308 kDa and is a glucosyltransferase, which irreversibly inactivates the Ras family of small GTPases to disrupt many important signalling pathways.
Clostridium difficile is a gram-positive motile bacterium which is the leading cause of diarrhoea in developed countries. The incidence of disease in humans varies greatly with age, spore density and the administration of antibiotics.
Toxin A (TcdA) is a large exotoxin which is an essential virulence factor for C. difficile infection. TcdA has a molecular mass of ~308 kDa and is a glucosyltransferase, which irreversibly inactivates the Ras family of small GTPases to disrupt many important signalling pathways.
Product Details
- Target Species
- Bacterial
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide (NaN3) - Immunogen
- Clostridium difficile toxin A.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
More Information
- UniProt
- P16154 Related reagents
- GO Terms
- GO:0009405 pathogenesis
- Regulatory
- For research purposes only
Applications of Clostridium difficile Toxin A antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/20 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Product Specific References
References for Clostridium difficile Toxin A antibody
-
Voth, D.E. & Ballard, J.D. (2005) Clostridium difficile toxins: mechanism of action and role in disease.
Clin Microbiol Rev. 18 (2): 247-63.
