IgG Antibody

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IgG Antibody gallery image 1

Published customer image:
Goat anti Monkey IgG antibody used for the evaluation of IgG responses in cynomolgus macaques following immunization by ELISA.
Image caption:
Serum and mucosally-detected anti-gp140 antibody titres after intravaginal immunisation followed by intramuscular immunisation (Group A). The intravaginal immunisation cycles, each consisting of 9× 100 µg gp140, are shown as broad stippled areas and intramuscular immunisation is indicated by a thin stippled line. For each animal, E53, E54, E55 and E56 results are shown for serum and cervical and vaginal fluids, with IgG indicated by filled symbols and IgA indicated by open symbols. Limits of detection for serum antibodies were titres of 100 and 10 for IgG and IgA respectively.

From: Cranage MP, Fraser CA, Cope A, McKay PF, Seaman MS, Cole T, Mahmoud AN, Hall J, Giles E, Voss G, Page M, Almond N, Shattock RJ. Antibody responses after intravaginal immunisation with trimeric HIV-1 CN54 clade C gp140 in Carbopol gel are augmented by systemic priming or boosting with an adjuvanted formulation.
Vaccine. 2011 Feb 4;29(7):1421-30.

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IgG Antibody gallery image 2

Published customer image:
Goat anti Monkey IgG antibody used for the evaluation of IgG responses in cynomolgus macaques following immunization by ELISA.
Image caption:
Serum and mucosally-detected anti-gp140 antibody titres after a single intramuscular immunisation followed by intravaginal immunisation (Group B) or a single intramuscular immunisation alone (Group D). The intramuscular immunisation is indicated by a thin stippled line and the intravaginal immunisation cycle, consisting of 9× 100 µg gp140, is shown as a broad stippled area. For each animal, E49, E50, E51, E52 (Group B) and E107 and E108 (Group D) results are shown for serum and cervical and vaginal fluids, with IgG indicated by filled symbols and IgA indicated by open symbols. Limits of detection for serum antibodies were titres of 100 and 10 for IgG and IgA respectively.

From: Cranage MP, Fraser CA, Cope A, McKay PF, Seaman MS, Cole T, Mahmoud AN, Hall J, Giles E, Voss G, Page M, Almond N, Shattock RJ. Antibody responses after intravaginal immunisation with trimeric HIV-1 CN54 clade C gp140 in Carbopol gel are augmented by systemic priming or boosting with an adjuvanted formulation.
Vaccine. 2011 Feb 4;29(7):1421-30.

Enlarge
IgG Antibody gallery image 3

Published customer image:
Goat anti Monkey IgG antibody used for the evaluation of IgG responses in cynomolgus macaques following immunization by ELISA.
Image caption:
Serum and mucosally-detected anti-gp140 antibody titres after three intramuscular immunisations followed by intravaginal immunisation (Group C). The intramuscular immunisations are indicated by thin stippled lines and the intravaginal immunisation cycle, consisting of 9× 100 µg gp140, is shown as a broad stippled area. For each animal, E57, E58, E59 and E60 results are shown for serum and cervical and vaginal fluids, with IgG indicated by filled symbols and IgA indicated by open symbols. Limits of detection for serum antibodies were titres of 100 and 10 for IgG and IgA respectively.

From: Cranage MP, Fraser CA, Cope A, McKay PF, Seaman MS, Cole T, Mahmoud AN, Hall J, Giles E, Voss G, Page M, Almond N, Shattock RJ. Antibody responses after intravaginal immunisation with trimeric HIV-1 CN54 clade C gp140 in Carbopol gel are augmented by systemic priming or boosting with an adjuvanted formulation.
Vaccine. 2011 Feb 4;29(7):1421-30.

Enlarge
IgG Antibody gallery image 4

Published customer image:
Goat anti Monkey IgG antibody used for the evaluation of IgG responses in cynomolgus macaques following immunization by ELISA.
Image caption:
Comparison of anti-gp140 specific antibodies and total immunoglobulins in vaginal and cervical secretions. Animals were immunised either once or three times by intramuscular immunisation with gp-140 in AS01 adjuvant followed by a cycle of 9 intravaginal immunisations with gp140 in Carbopol gel (Groups B and C) (a) The median titres of IgG and IgA antibodies were significantly different at the P = 0.006 (**) level for IgG but were not significantly different (ns) for IgA P = 0.097 (Mann–Whitney ranked sum test). (b) Total IgG concentrations were significantly higher in cervical secretions, P = 0.001 (**) (Mann–Whitney ranked sum test). (c) Total IgA concentrations in fluids from the two sites were not significantly different, P = 0.22. Group mean (- - -) and median (—) titres are shown with box plots.

From: Cranage MP, Fraser CA, Cope A, McKay PF, Seaman MS, Cole T, Mahmoud AN, Hall J, Giles E, Voss G, Page M, Almond N, Shattock RJ. Antibody responses after intravaginal immunisation with trimeric HIV-1 CN54 clade C gp140 in Carbopol gel are augmented by systemic priming or boosting with an adjuvanted formulation.
Vaccine. 2011 Feb 4;29(7):1421-30.

Enlarge
IgG Antibody gallery image 5

Published customer image:
Goat anti Monkey IgG antibody used for the evaluation of IgG responses in cynomolgus macaques following immunization by ELISA.
Image caption:
Correlation between virus neutralising activity and serum binding antibody titres. Neutralising activity was measured in sequential serum samples against tier 1 clade C HIV-1 MW963.26 pseudovirus and compared to serum IgG and IgA gp140 binding antibody titres from macaques immunised with gp140 by intravaginal-systemic (Group A) and systemic-intravaginal (Groups B and C) protocols.

From: Cranage MP, Fraser CA, Cope A, McKay PF, Seaman MS, Cole T, Mahmoud AN, Hall J, Giles E, Voss G, Page M, Almond N, Shattock RJ. Antibody responses after intravaginal immunisation with trimeric HIV-1 CN54 clade C gp140 in Carbopol gel are augmented by systemic priming or boosting with an adjuvanted formulation.
Vaccine. 2011 Feb 4;29(7):1421-30.

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  • IgG Antibody thumbnail image 1
  • IgG Antibody thumbnail image 2
  • IgG Antibody thumbnail image 3
  • IgG Antibody thumbnail image 4
  • IgG Antibody thumbnail image 5
  • Goat anti Monkey IgG (H/L):FITC
  • Goat anti Monkey IgG (H/L):Alk. Phos.
  • Goat anti Monkey IgG (H/L)
  • Goat anti Monkey IgG (H/L):HRP
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAI42FC, F, IC, IFdatasheet pdfdatasheet pdf1 mg
    AAI42F
    AAI42AEdatasheet pdfdatasheet pdf1 mg
    AAI42A
    AAI42E, IDdatasheet pdfdatasheet pdf1 mg
    AAI42
    AAI42PEdatasheet pdfdatasheet pdf1 mg
    AAI42P
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Monkey IgG (H/L) antibody recognizes monkey IgG (heavy and light chains). Goat anti Monkey IgG (H/L) antibody has been shown to react specifically with monkey IgG heavy chains and with light chains common to other monkey immunoglobulins by immunoelectrophoresis and ELISA. Reaction to human was also detected.

      Goat anti Monkey IgG (H/L) antibody may cross react with IgG from other species.
    • Intended Use
    • Target Species
      Monkey
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Humanyes
      African green monkey yes
      Cynomolgus monkeyyes
      Baboonyes
      Chimpanzeeyes
      Rhesus Monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Alkaline Phosphatase - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to monkey IgG were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.1% Proclin™ 300
      0.2% Bovine Serum Albumin
    • Immunogen
      Purified monkey IgG.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.
      DO NOT FREEZE.

      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch.
      18 months from date of despatch.
      12 months from date of despatch.
    • Acknowledgements
      Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/201/100
      Immunocytochemistry
      Immunofluorescence
      Immunohistology - Frozen1/201/100
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1,0001/10,000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/1000
      Immunodiffusion
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,0001/100,000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : HRP E 1 mg
    IgG Antibody : Purified E, ID 1 mg
    IgG Antibody : Alk. Phos. E 1 mg
    IgG Antibody : FITC C, F, IC, IF 1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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