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Leu-Enkephalin Antibody | NOC.1

Leu-Enkephalin Antibody | NOC.1 gallery image 1

Published customer image:
Mouse anti Leu-encephalin antibody, clone NOC.1 (4140-0355) used for the identification of Leu-encephalin expressing fibres in the porcine medulla oblongata by immunofluorescence.
Image caption:
a, FB-labeled neurons (arrows) in the porcine control DMX. b, GAL-IR fibers (arrowheads) in the DMX. c, GAL-IR fibers (arrowheads) in close proximity of the FB-labeled somata (arrows) in the DMX. d, FB-labeled neurons (arrows) in the porcine control DMX. e, SP-IR fibers (arrowheads) in the DMX. f, SP-IR fibers (arrowheads) surrounded the FB-labeled somata (arrows) enabling direct contact. g, FB-labeled neurons (arrows) in the porcine control DMX. h, LENK-IR fibers (arrowheads) in the DMX. i, LENK-IR fibers (arrowheads) formed basket-like structures encircling FB-labeled neurons (arrows) in the DMX. j, FB-labeled neuron (arrow) in the porcine control DMX. k, CART-IR fibers (arrowheads) in the DMX. l, CART-IR fibers (arrowheads) in close proximity of the FB-positive perikaryon (arrow) in the DMX. Photographs of Fig. c, f, i, l were prepared by superimposition of single image

From: Gańko M, & Całka J.
Prolonged acetylsalicylic-acid-supplementation-induced gastritis affects the chemical coding of the stomach innervating vagal efferent neurons in the porcine dorsal motor vagal nucleus (DMX).
J Mol Neurosci. 2014;54(2):188-98.

  • Leu-Enkephalin Antibody | NOC.1 thumbnail image 1
  • Mouse anti Leu-Enkephalin
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4140-0355C, Rdatasheet pdfdatasheet pdf0.1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human Leu5-enkephalin antibody, clone NOC.1 recognizes both Met5- and Leu5-enkephalin, secreted peptides which mimic the effect of opiate drugs. Mouse anti Human Leu5-enkephalin antibody, clone NOC.1 recognizes well established enkephalin immunoreactive sites, but does not bind to areas known to contain beta-endorphin or dynorphin in IHC.

      Cross reactivity with Met5-enkephalin and lack of reactivity with the peptide Gly-Gly-Phe-Leu and other related peptides by inhibition of radiolabelled Leu5-enkephalin binding is noted (Andersson et al. 1995).
    • Intended Use
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Tissue Culture Supernatant - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.05% Thiomersal
    • Immunogen
      Leu5-enkaphalin cinjugated to bovine serum albumin..
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      neuropeptide signaling pathway
      neuropeptide hormone activity
      extracellular region
      opioid peptide activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/1001/400

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Leu-Enkephalin Antibody Formats

    Formats Clone Applications Sizes available
    Leu-Enkephalin Antibody : S/N NOC.1 C, R 0.1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      Human anti Mouse IgG2a:FITCHCA037F0.1 mgF
      Human anti Mouse IgG2b:FITCHCA038F0.1 mgF
      Human anti Mouse IgG3:FITCHCA039F0.1 mgF
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      Human anti Mouse IgG2a:HRPHCA037P0.1 mgE
      Human anti Mouse IgG2b:HRPHCA038P0.1 mgE
      Human anti Mouse IgG3:HRPHCA039P0.1 mgE
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      Human anti Mouse IgG2b:RPEHCA038PE100 TestsF
      Human anti Mouse IgG3:RPEHCA039PE100 TestsF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Leu-Enkephalin Antibody | NOC.1 thumbnail image 1


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