Serine Protease Assay Kit
Product Details
- Reagents in the Kit
- FAM-Phe-DAP FLISP Reagent - lyophilized
10X Wash Buffer, 15 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst Stain, 1 mL - Test Principle
- Active chymotrypsin-like enzymes within the cell bind covalently to the FLISP FAM-Phe-DAP inhibitor probe, retaining the green fluorescent signal within the cell. The higher the concentration of active chymotrypsin-like enzymes within the cell, the higher the level of fluorescence observed. FLISP kits are suitable for use with suspension or adherent cells and can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader.
FLISP inhibitors are cell-permeable and non-cytotoxic fluorochrome-labeled analogs of the first serine protease inhibitor, tosyl-phenlalanylchloromethyl ketone (TPCK), labeled with carboxyfluorescein (FAM) which fluoresces green. They are available with either a chloromethyl ketone (CMK) or diphenyl 1-(N-peptidylamino) alkanephosphonate (DAP) reactive group containing compound. Specificity for chymotrypsin-like enzymes is determined by the amino acid targeting peptide, the FAM-Phe-DAP probe inhibits chymotrypsin.
Storage Information
- Storage
- MULTIPLE STORAGE CONDITIONS APPLY ON ARRIVAL. Store the unopened kit (and each unopened component) according to the storage instructions on each component label.
Store the FLISP reagent at -20oC. Once reconstituted in DMSO, the FLISP stock should be stored at -20oC for 6 months, protected from light. Avoid repeated freezing and thawing. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- Acknowledgements
- FLISP is a trademark of Immunochemistry Technologies, LLC.
- Regulatory
- For research purposes only
Applications of Serine Protease Assay Kit
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | |||
Immunofluorescence |
- Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/APO009.pdf
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
FAM FLICA™ Poly Caspase Kit | ICT091 | F IF | 25 Tests | ||
FAM FLICA™ Poly Caspase Kit | ICT092 | F IF | 100 Tests | ||
FLICA™ 660 Poly Caspase Kit | ICT9120 | F IF | 25 - 50 Tests | ||
SR FLICA™ Poly Caspase Kit | ICT916 | F IF | 25 Tests | ||
SR FLICA™ Poly Caspase Kit | ICT917 | F IF | 100 Tests |
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra