Poly Caspase
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IF ResetSR FLICA™ Poly Caspase Kit
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| The SR FLICA Poly Caspase Kit uses a quick and easy method to analyze active caspases in apoptotic cells. This FLICA reagent binds the following activated caspases: caspase 1, caspase 3, caspase 4, caspase 5, caspase 6, caspase 7, caspase 8 and caspase 9. It can also be used as a generic probe for the detection of most caspases. |
- Reagents in the Kit
- Pack Size: 25 Tests1 vial of SR-VAD-FMK FLICA Reagent - lyophilized
10x Apoptosis Wash Buffer, 15 mL
Fixative, 6 mL
Hoechst 33342, 1 mLPack Size: 100 Tests4 vials of SR-VAD-FMK FLICA Reagent - lyophilized
10x Apoptosis Wash Buffer, 60 mL
Fixative, 6 mL
Hoechst 33342, 1 mL - Test Principle
- Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a red (Sulforhodamine, SR) fluorescent probe.
The Caspase FLICA kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.
This kit can be used with a fluorescence microscope or a fluorescence plate reader using black microtitre plates. - Max Ex/Em
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Fluorophore Excitation Max (nm) Emission Max (nm) Sulforhodamine 565 590 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Acknowledgements
- FLICA™ is a trademark of Immunochemistry Technologies, LLC.
Store the unopened kit and each unopened component at +4oC until the expiration date.
Once reconstituted with DMSO, use FLICA reagent immediately, or store at -20oC for 6 months protected from light and thawed no more than twice during that time.
Once reconstituted with DMSO, use FLICA reagent immediately, or store at -20oC for 6 months protected from light and thawed no more than twice during that time.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
||
| Immunofluorescence | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT916.pdf
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT917.pdf
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| FAM FLICA™ Caspase-3/7 Kit | ICT093 | F IF | 25 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | FAM FLICA™ Caspase-3/7 Kit | ||||||
| FAM FLICA™ Caspase-6 Kit | ICT095 | F IF | 25 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | FAM FLICA™ Caspase-6 Kit | ||||||
| FAM FLICA™ Caspase-1 Kit | ICT097 | F IF | 25 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | FAM FLICA™ Caspase-1 Kit | ||||||
| FAM FLICA™ Caspase-8 Kit | ICT099 | F IF | 25 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | FAM FLICA™ Caspase-8 Kit | ||||||
| FAM FLICA™ Caspase-9 Kit | ICT912 | F IF | 25 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | FAM FLICA™ Caspase-9 Kit | ||||||
| SR FLICA™ Poly Caspase Kit | ICT917 | F IF | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | SR FLICA™ Poly Caspase Kit | ||||||
| FAM FLICA™ Caspase-2 Kit | ICT918 | F IF | 25 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | FAM FLICA™ Caspase-2 Kit | ||||||
| FAM FLICA™ Caspase-3/7 Kit | ICT094 | F IF | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | FAM FLICA™ Caspase-3/7 Kit | ||||||
| FAM FLICA™ Caspase-6 Kit | ICT096 | F IF | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | FAM FLICA™ Caspase-6 Kit | ||||||
| FAM FLICA™ Caspase-1 Kit | ICT098 | F IF | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | FAM FLICA™ Caspase-1 Kit | ||||||
| FAM FLICA™ Caspase-8 Kit | ICT910 | F IF | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | FAM FLICA™ Caspase-8 Kit | ||||||
| FAM FLICA™ Caspase-9 Kit | ICT913 | F IF | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | FAM FLICA™ Caspase-9 Kit | ||||||
| FAM FLICA™ Caspase-10 Kit | ICT923 | F IF | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | FAM FLICA™ Caspase-10 Kit | ||||||
References for Poly Caspase
-
Soni, C. et al. (2015) Distinct and synergistic roles of FcγRIIB deficiency and 129 strain-derived SLAM family proteins in the development of spontaneous germinal centers and autoimmunity.
J Autoimmun. 63: 31-46. -
Wong, E.B. et al. (2015) B cell-intrinsic CD84 and Ly108 maintain germinal center B cell tolerance.
J Immunol. 194 (9): 4130-43.
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