Poly Caspase
The SR FLICA Poly Caspase Kit uses a quick and easy method to analyze active caspases in apoptotic cells. This FLICA reagent binds the following activated caspases: caspase 1, caspase 3, caspase 4, caspase 5, caspase 6, caspase 7, caspase 8 and caspase 9. It can also be used as a generic probe for the detection of most caspases.
Product Details
- Reagents in the Kit
- Pack Size: 25 Tests1 vial of SR-VAD-FMK FLICA Reagent - lyophilized
10x Apoptosis Wash Buffer, 15 mL
Fixative, 6 mL
Hoechst 33342, 1 mLPack Size: 100 Tests4 vials of SR-VAD-FMK FLICA™ Reagent - lyophilized
10X Wash Buffer, 60 mL
Fixative, 6 mL
Hoechst Stain, 1 mL - Test Principle
- Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a red (Sulforhodamine, SR) fluorescent probe.
The Caspase FLICA kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.
This kit can be used with a fluorescence microscope or a fluorescence plate reader using black microtitre plates.
Storage Information
- Storage
- Pack Size: 25 TestsStore the unopened kit and each unopened component at +4oC until the expiration date.
Once reconstituted with DMSO, use FLICA reagent immediately, or store at -20oC for 6 months protected from light and thawed no more than twice during that time.Pack Size: 100 TestsStore the unopened kit (and each unopened component) at +4°C until the expiration date.
Protect the FLICA™ reagent from light at all times.
Once reconstituted, the 150X FLICA™ stock should be stored at -20°C protected from light. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- Acknowledgements
- FLICA™ is a trademark of Immunochemistry Technologies, LLC.
- Regulatory
- For research purposes only
Applications of Poly Caspase
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunofluorescence |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT916.pdf
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| FAM FLICA™ Caspase-3/7 Kit | ICT093 | F IF | 25 Tests | ||
| FAM FLICA™ Caspase-6 Kit | ICT095 | F IF | 25 Tests | ||
| FAM FLICA™ Caspase-1 Kit | ICT097 | F IF | 25 Tests | ||
| FAM FLICA™ Caspase-8 Kit | ICT099 | F IF | 25 Tests | ||
| FAM FLICA™ Caspase-9 Kit | ICT912 | F IF | 25 Tests | ||
| SR FLICA™ Poly Caspase Kit | ICT917 | F IF | 100 Tests | ||
| FAM FLICA™ Caspase-2 Kit | ICT918 | F IF | 25 Tests | ||
| FAM FLICA™ Caspase-3/7 Kit | ICT094 | F IF | 100 Tests | ||
| FAM FLICA™ Caspase-6 Kit | ICT096 | F IF | 100 Tests | ||
| FAM FLICA™ Caspase-1 Kit | ICT098 | F IF | 100 Tests | ||
| FAM FLICA™ Caspase-8 Kit | ICT910 | F IF | 100 Tests | ||
| FAM FLICA™ Caspase-9 Kit | ICT913 | F IF | 100 Tests | ||
| FAM FLICA™ Caspase-10 Kit | ICT923 | F IF | 100 Tests |
Product Specific References
References for Poly Caspase
-
Soni, C. et al. (2015) Distinct and synergistic roles of FcγRIIB deficiency and 129 strain-derived SLAM family proteins in the development of spontaneous germinal centers and autoimmunity.
J Autoimmun. 63: 31-46. -
Wong, E.B. et al. (2015) B cell-intrinsic CD84 and Ly108 maintain germinal center B cell tolerance.
J Immunol. 194 (9): 4130-43.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
