Mouse Ovalbumin Specific IgE ELISA Assay Kit

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Mouse Ovalbumin Specific IgE ELISA Assay Kit

Product Type
Kits
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2475KZZ E 96 Tests
Mouse Ovalbumin specific IgE ELISA Assay Kit accurately assays Ovalbumin specific IgE levels in a two-step direct ELISA using a rat anti-mouse IgE monoclonal antibody and HRP-conjugated Ovalbumin.

Product Details

Target Species
Mouse
Reagents in the Kit
Assay Buffer:

1 bottle (30 ml)

Detection Conjugate:

1 bottle (15 ml) HRP-labeled Ovalbumin, ready-to-use

Antibody-coated assay plate:

1 plate (96 wells) coated with rat anti-mouse IgE monoclonal antibody

Microplate for predispensing
samples:

1 plate (96 wells)

Wash Buffer Concentrate:

1 bottle (90 ml)

HRP Substrate:

1 bottle (15 ml), ready-to-use

Stop Solution:

1 bottle (15 ml), ready-to-use

Standards:6 vials

Storage Information

Storage
Store in a cool place( +4oC).
Avoid light and freezing.
Shelf Life
6 months from the date of despatch.

More Information

Regulatory
For research purposes only

Applications of Mouse Ovalbumin Specific IgE ELISA Assay Kit

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/MCA2475KZZ.pdf

Application Based External Images

ELISA

Product Specific References

References for Mouse Ovalbumin Specific IgE ELISA Assay Kit

  1. Hajek, A.R. et al. (2008) 12/15-Lipoxygenase deficiency protects mice from allergic airways inflammation and increases secretory IgA levels.
    J Allergy Clin Immunol. 122 (3): 633-9.e3.
  2. Aranzamendi, C. et al. (2012) Protection against allergic airway inflammation during the chronic and acute phases of Trichinella spiralis infection.
    Clin Exp Allergy. 43: 103-15.
  3. Naura, A.S. et al. (2013) Minocycline blocks asthma-associated inflammation in part by interfering with the T Cell receptor-NF-κB-GATA-3-IL-4 axis without a prominent effect on PARP.
    J Biol Chem. 288: 1458-68.
  4. Lee, M.Y. et al. (2013) Pinellia ternata Breitenbach attenuates ovalbumin-induced allergic airway inflammation and mucus secretion in a murine model of asthma.
    Immunopharmacol Immunotoxicol. 35 (3): 410-8.
  5. He, C. et al. (2013) Measles virus-derived peptide/food antigen adducts facilitate the establishment of antigen specific oral tolerance.
    J Physiol Pharmacol. 64 (1): 95-102.
  6. Toomer, O.T. et al. (2014) Maternal and postnatal dietary probiotic supplementation enhances splenic regulatory T helper cell population and reduces ovalbumin allergen-induced hypersensitivity responses in mice.
    Immunobiology. 219 (5): 367-76.
  7. Ghonim, M.A. et al. (2015) DNA-dependent protein kinase inhibition blocks asthma in mice and modulates human endothelial and CD4+ T-cell function without causing severe combined immunodeficiency.
    J Allergy Clin Immunol. 135 (2): 425-40.
  8. Chung, S.H. et al. (2015) The C-C Chemokine receptor 6 (CCR6) is crucial for Th2-driven allergic conjunctivitis.
    Clin Immunol. pii: S1521-6616(15)30021-8.
  9. Kwon, J.Y. et al. (2016) TRPV1 Antagonist Suppresses Allergic Conjunctivitis in a Murine Model.
    Ocul Immunol Inflamm. Oct 11 [Epub ahead of print]
  10. Lew, D.B. et al. (2017) Beneficial Effects of Prebiotic Saccharomyces cerevisiae Mannan on Allergic Asthma Mouse Models.
    J Immunol Res. 2017: 3432701.