Mitochondrial Membrane Potential MitoPT™ TMRM Kit

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Mito Membrane Potential MitoPT TMRM Kit gallery image 1 — Jurkat cells were cultured in the absence (healthy) or presence (dying) of staurosporine to induce apoptosis and then stained with TMRM dye. Cells were analyzed using a Nikon Eclipse E800 photomicroscope using a green excitation filter (510-560 nm) in tandem with a 570-620 nm emission filter. Apoptotic cells with depolarized mitochondria exhibit reduced orange/red fluorescence

Mitochondrial Membrane Potential Kit

Product Type: Kits

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
ICT948	F IF			500 Tests

Summary
Useful Reagents

The MitoPT™ TMRM kit uses a quick and easy staining method to clearly differentiate between non-apoptotic and apoptotic cells through mitochondrial functionality.

Product Details

Reagents in the Kit: MitoPT^TM TMRM Reagent - lyophilized - to make up 1mM stock
10X Assay Buffer, 125mL
600uL vial 50mM Carbonylcyanide m - chlorophenylhydrazone (CCCP) concentrate in DMSO.
Test Principle: An early indication of apoptosis involves a collapse in the electrochemical gradient across the mitochondrial membrane. Loss of mitochondrial membrane potential can be detected by a unique fluorescent cationic dye known as TMRM (tetramethylrhodamine methyl ester), that has been incorporated into the MitoPT^TM TMRM kit.

The MitoPT^TM TMRM reagent easily penetrates cells and enters the mitochondria. It aggregates in the mitochondria of non-apoptotic cells and fluoresces bright orange/red, whilst in apoptotic cells it diffuses throughout the cell. Once dispersed, the reagent assumes a monomeric form and exhibits a reduced orange/red fluorescence level. This allows an easy distinction between apoptotic and non-apoptotic fluorescent cells which can be read with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.

Storage Information

Storage: Store the unopened kit (and each unopened component) at -20^oC until the expiration date. Protect the MitoPT^TM TMRM reagent from light at all times.

Once reconstituted, the 1mM MitoPT^TM TMRM stock should be stored at -20^oC protected from light.
Shelf Life: Please see label for expiry date

More Information

Acknowledgements: MitoPT^TM is a trademark of Immunochemistry Technologies, LLC.
Regulatory: For research purposes only

Applications of Mitochondrial Membrane Potential MitoPT™ TMRM Kit

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry
Immunofluorescence

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Instructions For Use: Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/IFUICT948.pdf

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mitochondrial Membrane Potential Kit	ICT946	500 Tests	F IF

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