Histar Detection Kit

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Histar Detection System

Product Type
Kits
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
STAR3000A C P 50 Tests
STAR3000B C P 150 Tests
STAR3000C C P 500 Tests

Product Details

Intended Use
Bio-Rad HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining.

The HISTAR Detection System is designed for the staining of Human tissue sections. This kit is suitable for use with mouse and rabbit primary antibodies only.

All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of Bio-Rad ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete Bio-Rad PDF datasheet, that can be accessed by clicking on the "Instructions For Use" link. The procedure has been briefly summarized below:

1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary.
2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS).
3) Apply serum block and incubate for 15 minutes.
4) Apply primary antibody and incubate for 30 minutes.
5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash).
6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash).
7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.
[Discard unused substrate solution]
8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.

Reagents in the Kit
Pack Size: 50 Tests
VIAL 1, Serum Block
1 x 5ml, 5% Normal Serum in 0.01M Phosphate Buffered Saline (PBS) containing 0.1% Sodium Azide (NaN3)
VIAL 2, Reagent Boost
1 x 5ml
VIAL 3, HRP Polymer
1 x 5ml
VIAL 4, Peroxide Blocking Reagent
1 x 5ml, 0.03% Hydrogen Peroxide (H202)

VIAL A, DAB Chromogen
1 x 2ml, 2.5% 3,3’ Diaminobenzidine in stabilizing buffer
VIAL B, DAB Substrate Buffer
4 x 6ml, 0.1% H2O2 in Citric acid buffer

Pack Size: 150 Tests
VIAL 1, Serum Block
1 x 15ml

VIAL 2, Reagent Boost
1 x 15ml

VIAL 3, HRP Polymer
1 x 15ml
Pack Size: 500 Tests
VIAL 1, Serum Block
1 x 50ml

VIAL 2, Reagent Boost
1 x 50ml

VIAL 3, HRP Polymer
1 x 50ml
Additional Reagents Required
1) Primary antibody and positive control
2) Phosphate Buffered Saline (PBS), pH7.4 (+ 0.2)
3) Counterstain
4) Mounting media

Bio-Rad can supply a number of these products separately, please see the ‘Recommended Useful Reagents’ section.

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.
Shelf Life
Please see label for expiry date.

More Information

Regulatory
For research purposes only

Applications of Histar Detection Kit

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen Ready to use
Immunohistology - Paraffin Ready to use
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/STAR3000A.pdf

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
10X Phosphate Buffered Saline BUF036A 1 Litre E IY
Permanent Aqueous Mounting Medium BUF058A 30 ml C P
Permanent Aqueous Mounting Medium BUF058B 100 ml C P
Peroxide Blocking Reagent BUF017B 50 ml P
Permanent Aqueous Mounting Medium BUF058C 250 ml C P

Product Specific References

References for Histar Detection Kit

  1. Nishimoto, K. et al. (2010) Association of EMCN with Susceptibility to Rheumatoid Arthritis in a Japanese Population.
    J Rheumatol. 38: 221-8.
  2. Dedrick, G.S. et al. (2011) Immunohistochemical study of human costotransverse joints: A preliminary investigation.
    Clin Anat. 24 (6): 741-7.
  3. Alexopoulou, Z. et al. (2016) Deubiquitinase Usp8 regulates α-synuclein clearance and modifies its toxicity in Lewy body disease.
    Proc Natl Acad Sci U S A. Jul 21. pii: 201523597. [Epub ahead of print]