HIS-TAG Protein

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Proteus IMAC Mini Purification Kit

Product Type
Purification Kit
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PUR025 PP 4 Units
IMAC Ni-IDA binds to HIS-6 tagged proteins.

Recommended Reading - Proteus IMAC Purification Handbook.

Product Details

Preservative Stabilisers
Buffers contain 0.09% Sodium Azide (NaN3)
Resin contains 20%Ethanol
Reagents in the Kit
4 x Ni-IDA Spin Column Plugs
4 x Spin Columns
8 x 2.2 ml Centrifuge Tubes
4 x 10 kDa MWCO Ultrafiltration Spinners
1 x 60 ml of 5 x Buffer A
1 x 35 ml of 1 x Buffer B
Plug Insertion Tool
Instructions

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
Shelf Life
24 months from date of despatch.

More Information

Acknowledgements
His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of HIS-TAG Protein

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Protein Purification
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mycoplasma Removal Agent BUF035 5 ml TC
Mouse anti Histidine Tag MCA1396 1 mg E IP WB

Product Specific References

References for HIS-TAG Protein

  1. Lombardi, A. et al. (2010) Pichia pastoris as a host for secretion of toxic saporin chimeras.
    FASEB J. 24: 253-65.
  2. Inobe, T. et al. (2015) Artificial regulation of p53 function by modulating its assembly.
    Biochem Biophys Res Commun. 467 (2): 322-7.
  3. Inobe, T. & Nozaki, M. (2016) Proteasomal degradation of damaged polyubiquitin.
    Biochem Biophys Res Commun. pii: S0006-291X(16)30196-6. [Epub ahead of print]
  4. Inobe, T. & Nukina, N. (2016) Rapamycin-induced oligomer formation system of FRB-FKBP fusion proteins.
    J Biosci Bioeng. 122 (1): 40-6.