Endotoxin Removal High Capacity Kit

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Proteus MIDI Endotoxin Removal High Capacity Spin Column Pack

Product Type
Purification Kit
Product Code Applications Pack Size List Price Quantity
12 Units

This kit offers rapid, one step, high grade (>95%) removal of endotoxin from a wide range of protein solutions, with >90% protein recovery.

We recommend reading the Proteus Endotoxin Removal Kit Handbook prior to use.

Product Details

Preservative Stabilisers
94% Preservative base of resin containing:
20% Aqueous Ethanol
80% 0.1M Sodium Chloride
Reagents in the Kit
12 x Spin Column Plugs containing Endotoxin Binding Resin
12 x Midi High Capacity Spin Columns (20ml capacity)
12 x 50ml Centrifuge Tubes
Plug Insertion Tool
Instructions for use
Endotoxin Level
This kit can be used for samples with endotoxin loads < 30,000 EU, and is required where up to 1,000,000 EU. For endotoxin loads < 3,000 EU the Mini Endotoxin Removal kit is recommended.
Additional Reagents Required
0.2µm and 1.2µm Syringe Filter Units
Low Endotoxin Pre-equilibration Buffer e.g PBS pH7.4

Storage Information

Upon receipt of kit, remove resin plugs box and store this at +4oC.
Store remainder of kit at room temperature.
Guaranteed until date of expiry. Please see product label.

More Information

For research purposes only

Applications of Endotoxin Removal High Capacity Kit

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Protein Purification
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/PUR030.pdf

Product Specific References

References for Endotoxin Removal High Capacity Kit

  1. Negus, D. et al. (2015) Parenteral Administration of Capsule Depolymerase EnvD Prevents Lethal Inhalation Anthrax Infection.
    Antimicrob Agents Chemother. pii: AAC.01547-15.

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra