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Cathepsin L

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Magic Red™ Cathepsin L Kit thumbnail image 1 Magic Red™ Cathepsin L Kit thumbnail image 2
Magic Red™ Cathepsin L Kit gallery image 1
Fluorescence detection of Cathepsin L in Jurkat cells using Magic Red MR-(FR)2 reagent.
Magic Red™ Cathepsin L Kit gallery image 2
Fluorescence detection of Cathepsin L in THP-1 cells using Magic Red MR-(FR)2 reagent.

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Magic Red™ Cathepsin L Kit

Product Type
Kits
Specificity
Cathepsin L

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Product Code Applications Pack Size List Price Your Price Qty
ICT941
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF 25 Tests loader
List Price Your Price
loader
ICT942
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF 100 Tests loader
List Price Your Price
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The Magic Red Cathepsin L kit uses a quick and easy method to analyze intracellular cathepsin L protease activity in whole living cells.

Reagents In The Kit
ICT941: Magic Red Substrate (MR-FR2), 25 Tests - lyophilized
Hoechst 33342, 1 mL
Acridine Orange, 0.5 mL
ICT942: Magic Red Substrate (MR-FR2), 100 Tests - lyophilized
Hoechst 33342, 1 mL
Acridine Orange, 0.5 ml.
Test Principle
ICT941
Cathepsin Magic Red kits measure cathepsin L protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product.

The Magic Red reagent contains a cathepsin L target sequence peptide (FR)2 linked to a red (Cresyl Violet) fluorescent probe.

The Cathepsin Magic Red kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.

This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.
ICT942
Cathepsin Magic Red Kits measure cathepsin protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product.

The Magic Red reagent contains a Cathepsin L target sequence peptide (FR)2 linked to a red (Cresyl Violet) fluorescent probe.

The Cathepsin Magic Red kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.

This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.
Regulatory
For research purposes only.
Guarantee
Guaranteed until date of expiry. Please see product label.
Acknowledgements
Magic Red is a trademark of Immunochemistry Technologies, LLC.

Store the unopened kit and each unopened component at +4°C until the expiration date.
Once reconstituted with DMSO, use Magic Red reagent immediately, or store at -20°C for 6 months protected from light and thawed no more than twice during that time.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Instructions For Use
ICT941
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT941.pdf
ICT942
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT942.pdf

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Magic Red™ Cathepsin L Kit ICT942 IF 100 Tests
List Price Your Price
Description Magic Red™ Cathepsin L Kit
View more products with CATHEPSIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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