Caspase-3/7
The Magic Red Caspase-3/7 kit (ICT935) used for the demonstration of apoptosis in normal human bronchial epithelial cells by immunofluorescent microscopy.
Image caption:
C) Quantitation of apoptosis as measured by Caspase 3/7 Magic Red probe in NHBE cells, 3 hours post-treatment with either media only (control), 1 μM staurosporine, 20 μg/ml LL-37, PAO1 at 10:1 MOI, or PAO1 + LL-37. Data represent means +/- SEM from n = 3 independent experimental repeats, *** p <0.001, ** p<0.01, * p<0.05 by 2-way ANOVA with Bonferroni Post-test, or by unpaired t-test to evaluate the positive control staurosporine. D) Confocal microscopy images, at high and low power magnification, of NHBE cells treated with 20 μg/ml LL-37 and PAO1 at 10:1 MOI, co-stained with Caspase 1 FLICA probe (green) and Caspase 3/7 Magic Red probe (red), showing mutual exclusivity of cell staining with these probes. Representative of n = 4 separate experiments.
From: McHugh BJ, Wang R, Li H-N, Beaumont PE, Kells R, Stevens H, et al. (2019)
Cathelicidin is a “fire alarm”, generating protective NLRP3-dependent airway epithelial cell inflammatory responses during infection with Pseudomonas aeruginosa.
PLoS Pathog 15(4): e1007694.
doi: 10.1371/journal.ppat.1007694
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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IF ResetMagic Red™ Caspase-3/7 Kit
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- Kits
- Specificity
- Caspase-3/7
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| The Magic Red Caspase-3/7 kit uses a quick and easy method to analyze intracellular active caspases in apoptotic cells. |
- Reagents in the Kit
- Pack Size: 25 TestsMagic Red Substrate MR-(DEVD)2, 25 Tests - lyophilized
Hoechst 33342, 1 mL
Acridine Orange, 0.5 mLPack Size: 100 TestsMagic Red Substrate MR-(DEVD)2, 100 Tests - lyophilized
Hoechst 33342, 1 mL
Acridine Orange, 0.5 mL - Test Principle
- Caspase Magic Red kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of caspases to produce a fluorescent product. The Magic Red reagent contains a caspase inhibitor sequence (DEVD)2 linked to a red (Cresyl Violet) fluorescent probe.
The Caspase Magic Red kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.
This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates. - Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Acknowledgements
- Magic Red™ is a trademark of Immunochemistry Technologies, LLC.
Store the unopened kit and each unopened component at +4oC until the expiration date.
Once reconstituted with DMSO, use Magic Red reagent immediately, or store at -20oC for 6 months protected from light and thawed no more than twice during that time.
Once reconstituted with DMSO, use Magic Red reagent immediately, or store at -20oC for 6 months protected from light and thawed no more than twice during that time.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT935.pdf
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT936.pdf
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Magic Red™ Caspase-3/7 Kit | ICT936 | IF | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Magic Red™ Caspase-3/7 Kit | ||||||
References for Caspase-3/7
-
Wei, Z. et al. (2011) Notch activation enhances the microglia-mediated inflammatory response associated with focal cerebral ischemia.
Stroke. 42 (9): 2589-94. -
Shibata, Y. et al. (2016) Controlled in-cell release of caspase from photodegradable nanoparticles using the PARCEL method
Applied Spectroscopy Reviews. Mar 13 [Epub ahead of print] -
McHugh, B.J. et al. (2019) Cathelicidin is a "fire alarm", generating protective NLRP3-dependent airway epithelial cell inflammatory responses during infection with Pseudomonas aeruginosa..
PLoS Pathog. 15 (4): e1007694.
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