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Caspase-1

Product Code Applications Pack Size List Price Your Price Qty
ICT097
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 25 Tests loader
List Price Your Price
loader
ICT098
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 100 Tests loader
List Price Your Price
loader

The FAM FLICA Caspase-1 Kit uses a target sequence (YVAD) sandwiched between a green fluorescent label, carboxyfluorescein (FAM), and a fluoromethylketone (FMK) to make a quick and flexible method to analyze active caspases in apoptotic cells.

Reagents In The Kit
ICT097: 1 vial of FAM-YVAD-FMK FLICA Reagent - lyophilized
10x Apoptosis Wash Buffer, 15 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst 33342, 1 mL.
ICT098: 4 vials of FAM-YVAD-FMK FLICA Reagent - lyophilized
10x Apoptosis Wash Buffer, 60 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst 33342, 1 mL.
Test Principle
ICT097: Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.

The Caspase FLICA Kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.

This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.
ICT098: Caspase FLICA Kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.

The Caspase FLICA Kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.

This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FAM 494 520
Regulatory
For research purposes only.
Guarantee
Guaranteed until date of expiry. Please see product label.
Acknowledgements
FLICA is a trademark of Immunochemistry Technologies, LLC.

Store the unopened kit and each unopened component at +4°C until the expiration date.
Once reconstituted with DMSO, use FLICA reagent immediately, or store at -20°C for 6 months protected from light and thawed no more than twice during that time.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT097.pdf
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT098.pdf

Description Product Code Applications Pack Size List Price Your Price Quantity
FAM FLICA™ Caspase-1 Kit ICT098 F IF 100 Tests
List Price Your Price
Description FAM FLICA™ Caspase-1 Kit

References for Caspase-1

  1. Inokuchi, T. et al. (2006) Plasma interleukin (IL)-18 (interferon-gamma-inducing factor) and other inflammatory cytokines in patients with gouty arthritis and monosodium urate monohydrate crystal-induced secretion of IL-18.
    Cytokine. 33 (1): 21-7.
  2. Hoegen, T. et al. (2011) The NLRP3 Inflammasome Contributes to Brain Injury in Pneumococcal Meningitis and Is Activated through ATP-Dependent Lysosomal Cathepsin B Release.
    J Immunol. 187: 5440-51.
  3. Wang, Y. et al. (2012) A comparative study of stress-mediated immunological functions with the adjuvanticity of alum.
    J Biol Chem. 287 (21): 17152-60.
  4. Burm, S.M. et al. (2015) Inflammasome-induced IL-1β secretion in microglia is characterized by delayed kinetics and is only partially dependent on inflammatory caspases.
    J Neurosci. 35 (2): 678-87.
  5. Sharma, A.A. et al. (2015) Impaired NLRP3 inflammasome activity during fetal development regulates IL-1β production in human monocytes.
    Eur J Immunol. 45 (1): 238-49.
  6. Wang, Y. et al. (2015) Stress-activated Dendritic Cells (DC) Induce Dual Interleukin (IL)-15- and IL1β-mediated Pathways, Which May Elicit CD4+ Memory T Cells and Interferon (IFN)-stimulated Genes.
    J Biol Chem. 290 (25): 15595-609.
  7. Gabrion, A. et al. (2017) Mammalian target of rapamycin inhibition counterbalances the inflammatory status of immune cells in patients with chronic granulomatous disease.
    J Allergy Clin Immunol. 139 (5): 1641-9.e6.

Immunofluorescence

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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