Biotin (Type 2) Conjugation Kit

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LYNX Rapid Biotin (Type 2) Antibody Conjugation Kit

Product Type
Conjugation Kit
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
LNK192B CJ 1 Conjugation For 2mg Antibody
LYNX Rapid Biotin (Type 2) Antibody Conjugation Kit® enables the rapid conjugation of a pre-prepared lyophilized mixture containing Biotin label to an antibody or protein. Activation of proprietary reagents within the antibody-label solution results in the coupling of the antibody to Biotin, which has an extended spacer.

The LYNX Rapid Conjugation kit® can be used to label small quantities of antibody/protein at near neutral pH, allowing a high conjugation efficiency with 100% antibody recovery.

This kit has been optimized for use in assays in which the conjugate is captured by a Streptavidin labelled plate. For customers intending to complex their conjugates with a Streptavidin detection reagent, we recommend our LYNX Rapid Biotin (Type 1) Antibody Conjugation Kit.

Product Details

Reagents in the Kit
1 Vial of 1mg LYNX lyophilized Biotin mix
1 Vial LYNX Modifier reagent
1 Vial LYNX Quencher reagent
Preparing The Antibody
The following buffer solutions are recommended:for preparing the antibody:

10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

Do not use buffers containing nucleophilic components e.g primary amines and thiols since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

It is recommended that up to 2mg antibody be used in each labelling reaction. For optimal results the antibody volume should be 400-1000ul, at a concentration range equal to or greater than 1mg/ml.

Storage Information

Store kit at -20oC only.
Newly-conjugated antibody can be stored at 4oC. For long term storage however, the addition of a preservative is recommended.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing.
Shelf Life
12 months after despatch.

More Information

Licensed Use
Bio-Rad LYNX Rapid Conjugation kits are offered for research purposes alone, and are not intended for human, therapeutic or diagnostic use. The purchase of this conjugation kit conveys to the buyer (whether the buyer is a not-for-profit, academic or for-profit entity) the non-transferable right to use the amount of product purchased and the components of the product for in-house research. The buyer shall not sell or otherwise transfer this product, its components, or materials prepared therefrom to any third party. The buyer shall not use this product or its components for commercial purposes. For the avoidance of doubt, ?commercial purposes? means any activity by a party for consideration and includes, without limitation, use of the product or its components (i) in the manufacturing of conjugated materials (e.g. labeled antibodies), (ii) to provide a service, information or data, (iii) for therapeutic, diagnostic or prophylactic purposes, or (iv) for repackaging/resale, whether or not such product or its components are resold for use in research. The use of this product by the buyer constitutes agreement with the terms of this limited use label license for LYNX products.
For research purposes only

Applications of Biotin (Type 2) Conjugation Kit

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution

We recommend that for each conjugation the user determines the best antibody:conjugate ratio.
Instructions For Use
1. To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.

2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.

3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.

4. After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.