Biotin (Type 1) Conjugation Kit

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LYNX Rapid Biotin (Type 1) Antibody Conjugation Kit

Product Type
Conjugation Kit
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
LNK041B CJ 3 Conjugations For 200µg Antibody
LYNX Rapid Biotin (Type 1) Antibody Conjugation Kit® enables the rapid conjugation of a pre-prepared lyophilized mixture containing Biotin label to an antibody or protein. Activation of proprietary reagents within the antibody-label solution results in the coupling of the antibody to Biotin, which has an extended spacer.

The LYNX Rapid Conjugation kit® can be used to label small quantities of antibody/protein at near neutral pH, allowing a high conjugation efficiency with 100% antibody recovery.

This kit has been optimized for the additional use of a streptavidin labelled reagent. For customers intending to capture their conjugate on Streptavidin coated plates, we recommend our LYNX Rapid Biotin (Type 2) Antibody Conjugation Kit instead.

Product Details

Reagents in the Kit
3 Vials of 100ug LYNX lyophilized Biotin mix
1 Vial LYNX Modifier reagent
1 Vial LYNX Quencher reagent
Preparing The Antibody
The following buffer solutions are recommended:for preparing the antibody:

10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

It is recommended that 100-200ug antibody be used in each labelling reaction. For optimal results the antibody volume should be 40-100ul, at a concentration range of 1-4mg/ml.

Storage Information

Storage
Store kit at -20oC only.
Newly-conjugated antibody can be stored at 4oC. For long term storage however, the addition of a preservative is recommended.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing.
Shelf Life
12 months after despatch

More Information

Licensed Use
These products and the methodology of conjugation are patent protected under United Kingdom patent number 2446088 and associated international patent applications. The purchase of this product conveys to the buyer the limited, non exclusive non-transferable right (without the right to resell repackage or further sublicense) under these patents to use the product to make conjugates for research and development purposes only. The purchaser cannot sell or otherwise transfer this product, or its components, or materials or data made using this product, or its components to a third party. Further information on purchasing licenses for diagnostic and other uses may be obtained by contacting Bio-Rad, at. Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxon. OX5 1GE UNITED KINGDOM. Tel: +44 1865 852 700. E-mail: antibodies@bio-rad.com
Regulatory
For research purposes only

Applications of Biotin (Type 1) Conjugation Kit

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Conjugation

We recommend that for each conjugation the user determines the best antibody:conjugate ratio.
Instructions For Use
1. To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.

2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.

3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.

4. After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.

Product Specific References

References for Biotin (Type 1) Conjugation Kit

  1. Ho, A.S. et al (2010) Novel biomarkers predict liver fibrosis in hepatitis C patients: alpha 2 macroglobulin, vitamin D binding protein and apolipoprotein AI.
    J Biomed Sci. 17: 58.
  2. Levit-Zerdoun, E. et al. (2016) Survival of Ig╬▒-Deficient Mature B Cells Requires BAFF-R Function.
    J Immunol. 196 (5): 2348-60.