Annexin V Kit

Annexin V:Pe Assay Kit

Product Type
Kits
Specificity
Annexin V Kit

Product Code Applications Pack Size List Price Your Price Qty
ANNEX50PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 50 Tests
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Search for Batch Specific Datasheets

This test employs the property of Annexin V to bind to the membrane phospholipid phosphatidylserine (PS) in the presence of Ca2+. PS is exposed at the cell surface during the early stages of apoptosis. Detection of PS is a very sensitive method for detecting cells entering apoptosis, at a time point considerably ahead of nuclear changes such as DNA degradation.

The conjugation protocol used to prepare this product has not changed the native phospholipid binding properties of Annexin V. This protocol is designed to measure apoptosis easily and quickly in a sample of suspended cells.

View our complete list of formats and sizes of Annexin V kits

Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Reagents in the Kit
Annexin V:PE50 tests
7-AAD Viability Staining Solution100 tests
10X Binding Buffer30 ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
1) Dilute the10X Binding Buffer to 1X in distilled water (1 ml Binding Buffer + 9 ml distilled water).

2) Wash cells once in PBS by gentle shaking or pipetting up and down. Then wash once in 1X binding buffer.

3) Resuspend cells in 1X Binding Buffer, adjusting to a cell density of 1-5 x 106 cells/ml.

4) Add 5 μl of PE conjugated Annexin V to 100 μl of the cell suspension.

5) Mix and incubate for 10 to15 minutes at room temperature.

6) Wash cells in 1X Binding Buffer.

7) Resuspend cells in 200 μl of 1X Binding Buffer.

8) Add 5 μl of 7-AAD Viability Staining Solution and incubate for 5 minutes at room temperature.

9) Analyse by flow cytometry within 4 hours. Store at 2-8°C in the dark.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Annexin V:FITC Assay Kit ANNEX100F F 100 Tests
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Description Annexin V:FITC Assay Kit
Annexin V:APC Assay Kit ANNEX200APC F 200 Tests
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Description Annexin V:APC Assay Kit
Annexin V:Pe Assay Kit ANNEX200PE F 200 Tests
List Price Your Price
Description Annexin V:Pe Assay Kit
Annexin V:FITC Assay Kit ANNEX20F F 20 Tests
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Description Annexin V:FITC Assay Kit
Annexin V:FITC Assay Kit ANNEX300F F 300 Tests
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Description Annexin V:FITC Assay Kit
Annexin V:APC Assay Kit ANNEX50APC F 50 Tests
List Price Your Price
Description Annexin V:APC Assay Kit

UniProt
P08758
Entrez Gene
ANXA5
GO Terms
GO:0005509 calcium ion binding
GO:0007596 blood coagulation
GO:0004859 phospholipase inhibitor activity
GO:0007165 signal transduction
GO:0005544 calcium-dependent phospholipid binding
GO:0005737 cytoplasm
GO:0006916 anti-apoptosis
GO:0050819 negative regulation of coagulation
View more products with ANNEXIN V KIT specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"