Annexin V Kit

Annexin V:APC Assay Kit

Product Type
Kits
Specificity
Annexin V Kit

Product Code Applications Pack Size List Price Your Price Qty
ANNEX50APC
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 50 Tests
List Price Your Price
Search for Batch Specific Datasheets

This test employs the property of Annexin V to bind to the membrane phospholipid phosphatidylserine (PS) in the presence of Ca2+. PS is exposed at the cell surface during the early stages of apoptosis. Detection of PS is a very sensitive method for detecting cells entering apoptosis, at a time point considerably ahead of nuclear changes such as DNA degradation.

The conjugation protocol used to prepare this product has not changed the native phospholipid binding properties of Annexin V. This protocol is designed to measure apoptosis easily and quickly in a sample of suspended cells.

View our complete list of formats and sizes of Annexin V kits

Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Reagents in the Kit
Annexin V:APC50 tests
Propidium Iodide100 tests
10X Binding Buffer30 ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
APC 650 661
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
1) Dilute the10X binding buffer to 1X in distilled water (1 ml binding buffer + 9 ml distilled water).

2) Wash cells once in PBS by gentle shaking or pipetting up and down. Then wash once in 1X binding buffer.

3) Resuspend cells in 1X binding buffer, adjusting to a cell density of 1-5 x 106 cells/ml.

4) Add 5 μl of APC conjugated Annexin V to 100 μl of the cell suspension.

5) Mix and incubate for 10 to15 minutes at room temperature.

6) Wash cells in 1X binding buffer.

7) Resuspend cells in 200 μl of 1X binding buffer.

8) Add 5μl of Propidium Iodide staining solution.

9) Analyse by flow cytometry within 4 hours. Store at 2-8°C in the dark.

Description Product Code Applications Pack Size List Price Your Price Quantity
Annexin V:FITC Assay Kit ANNEX100F F 100 Tests
List Price Your Price
Description Annexin V:FITC Assay Kit
Annexin V:APC Assay Kit ANNEX200APC F 200 Tests
List Price Your Price
Description Annexin V:APC Assay Kit
Annexin V:Pe Assay Kit ANNEX200PE F 200 Tests
List Price Your Price
Description Annexin V:Pe Assay Kit
Annexin V:FITC Assay Kit ANNEX20F F 20 Tests
List Price Your Price
Description Annexin V:FITC Assay Kit
Annexin V:FITC Assay Kit ANNEX300F F 300 Tests
List Price Your Price
Description Annexin V:FITC Assay Kit
Annexin V:Pe Assay Kit ANNEX50PE F 50 Tests
List Price Your Price
Description Annexin V:Pe Assay Kit

UniProt
P08758
Entrez Gene
ANXA5
GO Terms
GO:0005509 calcium ion binding
GO:0007596 blood coagulation
GO:0004859 phospholipase inhibitor activity
GO:0007165 signal transduction
GO:0005544 calcium-dependent phospholipid binding
GO:0005737 cytoplasm
GO:0006916 anti-apoptosis
GO:0050819 negative regulation of coagulation
View more products with ANNEXIN V KIT specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"