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Luciferase antibody | LUC-Y

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MCA2076
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C E F * IF WB 0.1 mg loader
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Mouse anti Luciferase antibody, clone LUC-Y detects luciferase of the North American firefly Photinus pyralis in cells and tissues.

Target Species
Insect
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant luciferase expressed in E. coli.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c were fused with cells of the SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/5000
Flow Cytometry 1 10μg/ml
Immunofluorescence
Immunohistology - Frozen
Western Blotting 1/100
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
Western Blotting
Mouse anti luciferase antibody, clone LUC-Y detects a band of approximately 55 - 60 kDa with a sample of 1ug of purified luciferase from Photinus pyralis on a western blot.

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References for Luciferase antibody

  1. van Beusechem, V.W. et al. (2002) Efficient and selective gene transfer into primary human brain tumors by using single-chain antibody-targeted adenoviral vectors with native tropism abolished.
    J Virol. 76: 2753-62.
  2. Howarth, J.L. et al. (2007) Hsp40 molecules that target to the ubiquitin-proteasome system decrease inclusion formation in models of polyglutamine disease.
    Mol Ther. 15: 1100-5.
  3. Keravala, A. et al. (2008) Long-term transgene expression in mouse neural progenitor cells modified with phiC31 integrase.
    J Neurosci Methods. 173: 299-305.
  4. Zhao, D. et al. (2008) Antivascular effects of combretastatin A4 phosphate in breast cancer xenograft assessed using dynamic bioluminescence imaging and confirmed by MRI.
    FASEB J. 22: 2445-51.
  5. Zhou, H. et al. (2009) Dynamic near-infrared optical imaging of 2-deoxyglucose uptake by intracranial glioma of athymic mice.
    PLoS One. 4: e8051.
  6. Laakkonen, J.P. et al. (2009) Clathrin-independent entry of baculovirus triggers uptake of E. coli in non-phagocytic human cells.
    PLoS One. 4:e5093.
  7. Spiotto, M.T. et al. (2010) Imaging the unfolded protein response in primary tumors reveals microenvironments with metabolic variations that predict tumor growth.
    Cancer Res. 70: 78-88.

Immunofluorescence

RRID
AB_323475
UniProt
P08659
GO Terms
GO:0005777 peroxisome
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0055114 oxidation-reduction process
GO:0008218 bioluminescence
GO:0047077 Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing) activity

MCA2076

156909 161761 1702

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