Latest Immunology eNews

Welcome to our news page, bookmark this page to keep up-to-date with new products, pricing updates, promotions and resources.

September 12, 2016

Top tips when using F4/80 clone CI:A3-1 in flow cytometry

  • Crucial controls: Tissue macrophages can be autofluorescent and sticky so for F4/80 we suggest unstained, Fc blocks and isotype controls to improve your staining
  • Best practice sample prep for healthy cells can improve your F4/80 staining. Collagenase treatment, EDTA and live/dead stains will help with sample retrieval and improve your data. See our Flow Guide and recent webinar for more information
  • Gating strategies use other common myeloid markers such as CD11b, CD11c and Gr-1 with F4/80 to accurately gate and identify your cell population
  • Peer review references to confirm use of this antibody
  • Fluorophore choice: When choosing the fluorophore always consider the cell source, cell number and antigen expression levels to get the best staining. F4/80 is available with the following fluorophores

Color

Fluorophore

25 Tests

100 Tests

 

Pacific blue

MCA497PBT

MCA497PB

 

Alexa488

MCA497A488T

MCA497A488

 

PE

MCA497PET

MCA497PE

 

APC

MCA497APCT

MCA497APC

 

Alexa647

MCA497A647T

MCA497A647

 

Alexa700

MCA497A700T

MCA497A700

 

PEAlexa647

MCA497P647T

MCA497P647

 

PEAlexa750

MCA497P750T

MCA497P750

 

 

 

 

 

 

25µg

100µg

 

FITC

MCA497FT

MCA497F

 

August 23, 2016

CD45 and its isoforms

CD45 is a type I transmembrane consisting of two intracellular phosphatase domains, a transmembrane domain and an extracellular domain.

  • The extracellular domain contains three membrane proximal fibronectin type II repeats, a cysteine rich region and the variable N-terminal region
  • The N-terminal region is heavily glycosylated and contains a number of domains dependent on alternative splicing of exons 4, 5, 6 and possibly 7 of the CD45 gene

For identification purposes, the resulting proteins have suffixes consisting of letters describing the individual exons presents in the N-terminal. Letter A indicates exon 4 is present, letter B exon 5 and letter C exon 6.

Read our new mini-review for detailed information on what isoforms have been identified and isolated, plus details of the antibodies that detect these isoforms.

 

August 15, 2016

F4/80 and myeloid cell resources

F4/80 and myeloid cell resources

Learn more about the definitive macrophage marker: F4/80 clone CI:A3-1

Monocyte, macrophage and dendritic cells development, lineage, characterization, key markers and antibodies, discover this complete resource.

Human and mouse specific immune cell lineage posters, cellular marker expression posters and comprehensive markers and antibody guides.

 

 

July 18, 2016

Gr-1 A647 (MCA2387) v CD11b PE (MCA711)

Murine bone marrow stained with Gr-1 A647 (MCA2387) and CD11b PE (MCA711) in the presence of murine SeroBlock (BUF041A).

Granulocytes research:
Neutrophils-Eosinophils-Basophils-Mast cells


Bio-Rad has a range of resources to aid your research and the selection of the right target marker and antibody.

  • Cell specific quick reference reviews
  • Identification of key markers per cell type
  • Antibodies against these key markers, plus many more against numerous granulocyte targets
  • Application specific tips and guides, helping you get the best experimental result
  • Human and mouse immune cell lineage and marker expression posters
     

June 27, 2016

A range of new B cell resources:

  • B cell overview: from development to activation and immune response. Plus antibodies against key markers.
  • B cell mini-review: detailed review of B cells including their role in cancer as their exploitation as a therapeutic agent.
  • Lineage posters: explore the development of human and murine B cells.
  • Marker guide: scientifically relevant information regarding key human and murine B cell markers
  • Acute myeloid leukemia focus: poster and research reagents

Also take a look at our featured markers: CD19, CD20, CD45 and CD52

May 31, 2016

Immune Biomarkers: A New Resource

We have introduced two new biomarker expression posters providing a cellular expression profile for over 130 top immune markers.

Designed for both the human and mouse immune systems they aid researchers in the quick and easy identification of more than 30 immune cell types.

 

May 5, 2016

Macrophages - Monocytes - Dendritic Cells

Monocyte development, characterization and role in disease Mini-review A complete resource

Discover more about these important myeloid immune cells:

  • Cell specific quick reference overviews
  • In depth macrophage and monocytes mini-reviews; focusing on cell development, polarization, functions and key markers
  • Identification of key markers
  • Broad choice of highly cited antibodies against these specific cell type markers

And don’t forget to check out our blogs:

Inducing macrophages that work against rather than for cancer” and coming soon “monocytes and disease.”

 

April 19, 2016

New! Human and murine immune cell marker guides and posters

New! Human and murine immune cell marker guides and posters To simplify marker selection we have developed a set of handy resources including posters and booklets for human and mouse immune cell markers.

Please select the resource of interest below for more details:


 


 


 

April 15, 2016

Lineage biomarkers of human immune system booklet –get your free copy today

Lineage biomarkers of human immune system booklet - get your free copy today

Simplifies human marker selection by compiling in one place:

  • Development of mature human immune cells from their precursors
  • Signature biomarkers (both CD and non-CD) specific to each immune cell type
  • Experimentally relevant information regarding each marker
  • Antibodies available for each marker
  • The booklet also includes a handy biomarker expression patterns grid enabling cell marker identification at a glance

February 23, 2016

Lineage biomarkers of murine immune system booklet – get your free copy today

Lineage biomarkers of murine immune system booklet - get your free copy today

Simplifies mouse marker selection by compiling all in one place:

  • Development of mature mouse immune cells from their precursors
  • Signature biomarkers (both CD and non-CD) specific to each immune cell type
  • Experimentally relevant information regarding each marker
  • Antibodies available for each marker
  • The booklet also includes a handy biomarker expression patterns grid enabling cell marker identification at a glance

January 25, 2016

Marker selection for human immune cells made easy – download or order today

Marker selection for human immune cells made easy – download or order today

Bio-Rad has developed a human lineage poster that details markers for each immune cell type.

  • Presented in a genealogical design 
  • Markers for myeloid cells, lymphocytes and stem cells
  • Colour coded according to the exact cell types
  • Easy to follow 
  • Makes finding your marker simple

December 1, 2015

Biomakers of transplantation rejection and C4d

As a marker of complement activation, C4d has been used widely as an indicator of acute antibody mediated rejection. There are many markers that can be used as an indicator of rejection.

To aid marker selection we have developed a table that lists a number of proteins that have been associated with transplantation along with the antibodies that we have against those protein markers. Also included are the cell types and organs that these proteins are expressed in.

November 16, 2015

Marker selection for mouse immune cells made easy – download or order today

Marker selection for mouse immune cells made easy – download or order today

Bio-Rad has developed a mouse lineage poster that details markers for each immune cell type.

  • Simplifies your marker selection
  • Easy to follow genealogical design
  • Color coded according to the exact cell types Includes markers for myeloid cells, lymphocytes and stem cells

November 11, 2015

Complement mini-review – download today

To help you gain a better understanding of the role of complement in Innate Immunity we have developed a mini-review which details the three main pathways, the role of inflammatory mediators and a focus on C4d.

Complement antibodies and proteins

Bio-Rad offers a continually evolving range of antibodies and recombinant proteins for use in complement research;

Complement product fact sheet

Complement product fact sheet

  Download PDF

  • Markers for the classical, alternative and MBL pathways
  • Receptor and regulatory protein antibodies
  • Antibodies to key targets such as C4d and C1q

For an overview of the product range, download your complimentary copy of the complement product fact sheet today

October 19, 2015

Dot Blot showing FSC/SSC gated rat peritonial macrophages dual stained with CD68 (MCA341A488) at neat and CD163 (MCA342A647) at a 1/5 dilution.

Two distinct populations of CD68 positive macrophages were observed, CD163-low M1 macrophages and CD163-high M2 macrophages.

Isotype control pair in red. Permeabilization was with Leucoperm™ (BUF09).

Innate Immunity cell markers for Flow Cytometry

Flow Cytometry can be a challenge in a number of ways, from experimental design to sample preparation. To help you overcome these challenges we have combined practical help and advice alongside marker selection.

Innate Immunity cell and cell receptor markers range

Along with general overviews we also provide cell or marker specific mini-reviews such as F4/80, NK cell and the ever popular macrophage polarisation mini-review.

Flow cytometry resources – practical help and advice 

For flow cytometry we can provide:





Leucoperm™ is made for Bio-Rad by AN DER GRUB Bio Research GmbH.

Sept 11, 2015

F4/80 (clone CI:A3-1) the murine macrophage marker of choice - use it in conjunction with other cell surface markers to identify various myeloid derived lineages

Earlier we revealed that our F4/80 antibody (clone CI:A3-1) is the definitive and most widely referenced in mouse macrophage research, used in almost 1000 publications. F4/80 (clone CI:A3-1) can be used in a wide range of applications; here we show how liver Kupffer cells/macrophages can be split into 3 distinct cell types, with functional and developmental differences using other common markers.

F4/80 +/CD32+/ CD68 + resident precursors which develop in the liver, rarely seen in the blood or spleen. Express other precursor cell surface markers such as c-kit and CD34.

F4/80 +/ CD68 + large spindle shaped cells matured in the liver. They phagocytose bacteria, killing them using reactive oxygen species, but do not release cytokines.

F4/80 +/ CD11b + recruited to the liver from the bone marrow, these small round cells are potent cytokine producers lacking the ability to effectively kill bacteria. These cytokines in turn activate NK cells for efficient anti-tumor activity.

The expression of CD68 and CD11b by liver F4/80+ Kupffer cells.

Liver MNCs were obtained from CD, HFD, HCD and HFCD mice, and Kupffer cells gated by F4/80 were analyzed for their expression levels of CD68 and CD11b. CD68 positive gate was determined by using the isotype control Ab. The numbers are the means ±SE from six to eight mice in each group. *P<0.05 vs. CD and HFD.

Reference: Nakashima H, et al. (2013). Activation of CD11b+ Kupffer Cells/Macrophages as a Common Cause for Exacerbation of TNF/Fas-Ligand-Dependent Hepatitis in Hypercholesterolemic Mice. PLoS ONE 8(1): e49339.

Aug 28, 2015

Discover what Innate Immunology antibodies Bio-Rad can offer you

Download Innate Immunity product brochures

We know how difficult it can be to find the right antibodies and reagents for your application. With this in mind we have developed a range of new product fact sheets to help you easily find the right products that meet your specificity, application and format requirements.

You can also pick up your free copies from any of the trade shows we are exhibiting at this year, to see the full list of exhibitions please click here .

Focus areas include:

Research Areas: Cell Types:
Apoptosis & Cell Viability Dendritic Cells
Complement System Macrophage Markers
Cytokines & Chemokines NK Cells
Innate Immunity Range  
Pattern Recognition Receptors (PRR)
July 16, 2015

F4/80 (clone CI:A3-1) the murine macrophage marker of choice – but don’t take our word for it – choose from hundreds of citations

Liver tissue stained with the F4/80 antibody MCA497

Liver tissue was fixed and then stained with MCA497 after pre-treatment with citrate buffer at pH 6.0 (view the full  F4/80 antigen retrieval technique ). Brown patches represent stained macrophages.

Mouse peritoneal macrophages dual stained with F4/80 antibody FITC

Dot Blot showing FSC/SSC gated mouse peritoneal macrophages dual stained with F4/80 antibody, FITC  (MCA497F) at a 1/5 dilution and  CD11b antibody  (MCA74A647) at a 1/5 dilution. Isotype control pair in red. Fc receptors were blocked by mouse Seroblock  (BUF041B)

F4/80 CI:A3-1 is the definitive and most widely referenced antibody clone in mouse macrophage research, used in almost 1000 publications. Our F4/80 antibodies ( MCA497 ) are performance guaranteed in a wide range of applications including Flow Cytometry, Immunohistochemistry, Western Blotting, Immunoprecipitation and Immunofluorescence/Immunocytochemistry.

To aid your research and understanding of this marker, a selection of F4/80 references is available, providing information about detailed methods, uses and advantages of our F4/80 antibody. Further references can be found on  CiteAb , where there are over 900 F4/80 clone CI:A3-1 citations. Finally, a mini-review providing a technical overview of the F4/80 antigen and antibody is also available to download.

July 8, 2015

Identify and quantify Mouse Fc receptor (FcR) expression

Complete range of Mouse FcR antibodies

  • Encompasses all four distinct Mouse FcR’s
  • Suitable for Flow Cytometry and Immunohistochemistry-Frozen applications
  • Mouse FcRs are classified as either activating or inhibitory:

Activating Mouse FcR

Mouse FcR CD Clone Product Code
FcRI CD64 AT152-9 MCA5997
FcRIII CD16 AT154-2 MCA5998
FcRIV CD16-2 AT137 MCA5999

Inhibitory Mouse FcR

Mouse FcR CD Clone Product Code
FcRIIB CD32B AT130-5 MCA6000
FcRIIB CD32B AT130-5R MCA6001

Click here for further details on this range, Mouse FcR classification and references.

June 16, 2015

New lower prices for favorite CD68 antibody formats

CD68 a pan-macrophage marker – an essential tool in macrophage research

New lower prices for your favorite CD68 rat anti-mouse antibody formats:

Format
Size
Product Code
FITC 25µg MCA1957FT
FITC 50µg MCA1957FA
FITC 100µg MCA1957F
PE 25 Tests MCA1957PET
PE 100 Tests MCA1957PE
PE 500 Tests MCA1957PEB
June 1, 2015

Murine macrophage marker F4/80 mini-review

F4/80 Antibody Mini Review - The marker and the antibody

F4/80 Antibody Mini Review - The marker and the antibody

  Download PDF

The F4/80 mini-review provides background information regarding the F4/80 antigen, the CI:A3-1 clone and the antibody. Specifically the F4/80 mini-review covers the following areas:

  • F4/80 gene and protein
  • F4/80 functions
  • F4/80 expression
  • F4/80 microglia
  • EMR1 the human homolog of F4/80
  • References

Don’t forget to check out the new lower prices of four popular formats of F4/80 antibodies ( MCA497 ) listed below.

May 13, 2015

Cutting the prices of popular F4/80 antibody formats

The definitive murine macrophage marker

Price reductions are for the four most frequently used F4/80 antibody formats:

Format
Size
Product Code
FITC 25μg MCA497FT
FITC 50μg MCA497FA
FITC 100μg MCA497F
FITC 500μg MCA497FB
Biotin* 100μg MCA497B
Biotin 500μg MCA497BB
PE* 100μg MCA497PE
APC 25μg MCA497APCT
APC 100μg MCA497APC

*other sizes also available