Browsing Modes for Business Users

Infliximab antibody | AbD20436

Human anti Infliximab

Product Type
Monoclonal Antibody
Clone
AbD20436
Isotype
HuCAL Fab monovalent
Specificity
Infliximab

Product Code Applications Pack Size List Price Your Price Qty
HCA234
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Human anti Infliximab, clone AbD20436, is an affinity matured anti-idiotypic antibody in monovalent Fab format that specifically recognizes free infliximab but not infliximab bound to its target, Tumor Necrosis Factor Alpha (TNFα). It can be used in bioanalytical assays to measure the levels of infliximab and biosimilar products, such as Inflectra and Renflexis, in patient samples.

Human anti Infliximab, clone AbD20436 inhibits the binding of infliximab to TNFα and can be used as a capture antibody in a pharmacokinetic (PK) assay in the bridging format to measure free drug.

Infliximab (branded as Remicade®) is a chimeric IgG1 kappa monoclonal antibody approved for the treatment of psoriasis, Crohn's disease, ankylosing spondylitis, psoriatic arthritis, rheumatoid arthritis and ulcerative colitis. Infliximab is directed against TNFα and acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.

View a summary of all anti-infliximab antibodies.

Product Form
A monovalent human recombinant Fab selected from the HuCAL? phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Source
E.coli
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Infliximab.
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=0.12 nM by real time, label-free molecular interaction analysis on immobilized infliximab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Remicade is a registered trademark of Janssen Biotech Inc. Inflectra is a registered trademark of Hospira, a Pfizer Company. RENFLEXIS is a trademark of Merck Sharp & Dohme Corp.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in an indirect ELISA system or as a capture reagent in PK assay development together with HRP-conjugated HCA215 or HCA233 as the detection reagent.
Protocol: PK bridging ELISA to measure free drug

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Human IgG F(ab')2:HRP 0500-0099 E WB 0.5 ml loader
List Price Your Price
loader
Description Goat anti Human IgG F(ab')2:HRP
Rabbit anti DYKDDDDK Tag:HRP AHP1074P E WB 0.1 mg loader
List Price Your Price
loader
Description Rabbit anti DYKDDDDK Tag:HRP
Goat anti Histidine Tag:Biotin AHP1656B E WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Histidine Tag:Biotin
Mouse anti Histidine Tag:Alk.Phos. MCA1396A E WB 0.1 mg loader
List Price Your Price
loader
Description Mouse anti Histidine Tag:Alk.Phos.
Mouse anti Histidine Tag:Biotin MCA1396B E WB 0.1 mg loader
List Price Your Price
loader
Description Mouse anti Histidine Tag:Biotin
Rat anti DYKDDDDK Tag:HRP MCA4764P E P WB 0.1 mg loader
List Price Your Price
loader
Description Rat anti DYKDDDDK Tag:HRP
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader
List Price Your Price
loader
Description Mouse anti Penta Histidine Tag:HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
Human anti Infliximab HCA213 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Infliximab
Human anti Infliximab HCA215 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Infliximab
Human anti Infliximab HCA233 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Infliximab
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
List Price Your Price
Description LYNX Rapid HRP Antibody Conjugation Kit
Recombinant Human TNF Alpha PHP051 E FN WB 50 µg
List Price Your Price
Description Recombinant Human TNF Alpha

Synonyms
REMICADE®
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

HCA234

164592 1806

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with INFLIXIMAB specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

Please note