Infliximab Antibody | AbD20436

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Infliximab Antibody | AbD20436 gallery image 1

Human anti infliximab specificity ELISA

A microtiter plate was coated over night with various antigens at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti infliximab (clone 20436) at a concentration of 2 µg/ml followed by an HRP-labelled anti-His tag in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate

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Human anti infliximab specificity titration ELISA

A microtiter plate was coated over night with human IgG1/kappa, human IgG1/lambda, cetuximab or infliximab at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti infliximab (clone AbD20436) titrated to the given concentrations in PBST+10% human serum, followed by an HRP-labelled anti-His tag in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate

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Infliximab bridging ELISA for Pharmacokinetic (PK) assay development

A microtiter plate was coated over night with Human anti infliximab (clone AbD20436) at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of infliximab. Detection was performed using HRP-conjugated Human anti infliximab (clone 19370_hIgG1, HCA215) in HISPEC assay diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®Enlarge

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Infliximab bridging ELISA for Pharmacokinetic (PK) assay development

A microtiter plate was coated over night with Human anti infliximab (clone AbD20436) at a concentration of 0.6 µg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of infliximab. Detection was performed using HRP-conjugated Human anti infliximab (clone AbD20436_hIgG1, HCA233) in HISPEC assay diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®Enlarge

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Human anti infliximab inhibits the binding of infliximab to TNFα

A microtiter plate was coated over night with TNFα at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, a pre-incubated mixture of infliximab (IFX, 0.3 µg/ml) plus increasing concentrations of Human anti infliximab (clone AbD20436) was added. Free infliximab, still capable of binding to the TNFα-coated plate, was detected using Goat anti Human IgG F(ab')2:HRP (0500-0099)

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  • Human anti Infliximab
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-infliximab antibody is a recombinant, neutralizing, high affinity anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assays for infliximab and biosimilars. It is suitable as the capture antibody in a PK bridging ELISA.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD20436
    • Isotype
      HuCAL Fab monovalent
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA234Edatasheet pdfdatasheet pdf0.1 mg
      HCA234
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Human anti Infliximab, clone AbD20436, is an affinity matured anti-idiotypic antibody in monovalent Fab format that specifically recognizes free infliximab but not infliximab bound to its target, Tumor Necrosis Factor Alpha (TNFα). It can be used in bioanalytical assays to measure the levels of infliximab and biosimilar products in patient samples.

        Clone AbD20436 inhibits the binding of infliximab to TNFα and can be used as a capture antibody in a pharmacokinetic (PK) assay in the bridging format to measure free drug.

        Infliximab (branded as Remicade®) is a chimeric IgG1 kappa monoclonal antibody approved for the treatment of psoriasis, Crohn's disease, ankylosing spondylitis, psoriatic arthritis, rheumatoid arthritis and ulcerative colitis. Infliximab is directed against TNFα and acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.

        View a summary of all anti-infliximab antibodies.
      • Intended Use
      • Product Form
        A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Metal chelate affinity chromatography
      • Preservative Stabilisers
        0.01% Thiomersal
      • Immunogen
        Infliximab.
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=0.12 nM by real time, label-free molecular interaction analysis on immobilized infliximab.
      • Approx. Protein Concentrations
        Antibody concentration 0.5 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany, a wholly owned subsidiary of Bio-Rad Laboratories Inc.

        Remicade is a registered trademark of Janssen Biotech Inc.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used in an indirect ELISA system or as a capture reagent in PK assay development together with HRP-conjugated HCA215 or HCA233 as the detection reagent.
        Protocol: PK bridging ELISA to measure free drug
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Infliximab Antibody Formats

      Formats Clone Applications Sizes available
      Infliximab Antibody : Purified AbD20436 E 0.1 mg
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse anti Histidine Tag:Alk.Phos.MCA1396A0.1 mgE, WB
        MCA1396A
        Goat anti Histidine Tag:BiotinAHP1656B0.1 mgE, WB
        AHP1656B
        Mouse anti Histidine Tag:BiotinMCA1396B0.1 mgE, WB
        MCA1396B
        Rabbit anti DYKDDDDK Tag:HRPAHP1074P0.1 mgE, WB
        AHP1074P
        Rat anti DYKDDDDK Tag:HRPMCA4764P0.1 mgE, P, WB
        MCA4764P
        Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
        0500-0099
        Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
        MCA5995P

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            LYNX Rapid HRP Antibody Conjugation KitLNK002P3 Conjugations For 400µg AntibodyCJ
            LNK002P
            Human anti InfliximabHCA2130.1 mgE
            HCA213
            Human anti InfliximabHCA2150.1 mgE
            HCA215
            Human anti InfliximabHCA2330.1 mgE
            HCA233
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A
            Recombinant Human TNF AlphaPHP05150 µgE, FN, WB
            PHP051

            Recommended Positive Controls

              Histology Controls

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