IgG/Rf Stripper

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  • IgG/Rf Stripper
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  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF059IYdatasheet pdfdatasheet pdf10 ml
    BUF059
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Intended Use
      High levels of IgG may mask low levels of IgM in patient sera, resulting in false negatives in certain assays. IgM class rheumatoid factor reacts with IgG immune complexes, leading to the production of pathogen specific immune complexes that can also interfere in certain assays.

      BUF059 is a sheep antiserum monospecific to human IgG. It is designed to remove IgG and rheumatoid factor of IgM class from human serum prior to testing for IgM. Patient samples to be tested for pathogen-specific IgM are diluted in IgG/RF stripper, allowing immune complexes to form containing patient IgG. Rheumatoid factor IgM will bind to these complexes, resulting in the removal of both pathogen specific IgG and rheumatoid factor IgM.

      IgG/RF stripper will remove IgG at concentrations of up to 15g/L IgG from human serum when used as directed.
    • Product Form
      Sheep antiserum monospecific to human IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.1% EACA
      0.01% Benzamidine
      1mM EDTA
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Glycine buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
    • Shelf Life
      Please see label for expiry date.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunoassay

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      Specimen collection and preparation

      Blood samples should be collected by venapuncture, allowed to clot naturally and the serum separated as soon as possible.
      Avoid using haemolysed, lipaemic or bacterially contaminated sera.
      Serum samples may be stored at 2-8°C for up to 72 hours. For longer-term storage, freeze at -20°C. Avoid repeated freezing and thawing.

      Protocol

      1. To one volume of serum sample (eg. 50ul), add four volumes of BUF059 (eg. 200ul).

      2. Mix and incubate at room temperature for 30 minutes.

      3. Centrifuge to remove precipitate.

      4. Serum sample is now ready for testing.

      5. Note: BUF059 will remove IgG at concentrations of up to 15g/L from human serum when used as directed above. Serum samples containing higher levels of IgG may require the addition of more IgG/RF stripper.

    Additional IgG/Rf Stripper Formats

    Formats Applications Sizes available
    IgG/Rf Stripper : Ready To Use IY 10 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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