Huntingtin Antibody | HDA3E10

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Huntingtin Antibody | HDA3E10 gallery image 1

Total protein extract of normal human cerebral cortex separated as a strip on a 3-12.5% gradient SDS-PAGE gel and Western blotted. The blot was probed with MCA2049

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  • Huntingtin Antibody | HDA3E10 thumbnail image 1
  • Mouse anti Huntingtin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    HDA3E10
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2049C *, IP, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2049
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Huntingtin antibody, clone HDA3E10 reacts with an epitope corresponding to the HDA region (amino acids 1171 - 1177) of the huntingtin protein.

      Mouse anti Huntingtin antibody, clone HDA3E10 detects a 350KD band on western blots but also detects smaller degradation products of huntingtin.

      The combined use of clone HDA3E10 (MCA2049) HDC8A4 (MCA2051) and HDB4E10 (MCA2050) demonstrate that huntingtin is enriched in neuronal cells in the brain.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunoprecipitation
      Western Blotting
      Immunohistology - Frozen(1)
      (1)
      Increased cytoplasmic staining, relative to nuclear, has been reported using formaldehyde as a fixative compared with acetone/methanol, see Wilkinson et al.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Brain
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Huntingtin Antibody Formats

    Formats Clone Applications Sizes available
    Huntingtin Antibody : Purified HDA3E10 C *, IP, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

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          Recommended Positive Controls

            Histology Controls

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              References

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