ZNF124 Antibody | 4G4

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ZNF124 Antibody | 4G4 gallery image 1

Western blot analysis of ZNF124 expression in Raw 2647 using Mouse anti Human ZNF124:Azide Free (MCA4195Z)

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  • ZNF124 Antibody | 4G4 thumbnail image 1
  • Mouse anti Human ZNF124:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
    4G4
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4195ZWBdatasheet pdfdatasheet pdf0.1 mg
    MCA4195Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human ZNF124 antibody, clone 4G4 recognizes human Zinc finger protein 124, also known as ZNF124 or Zinc finger protein HZF-16. ZNF124 is a 351 amino acid ~42 kDa nuclear DNA binding protein likely to be involved in transcriptional regulation.

      Multiple isoforms of ZNF124 generated by alternative splicing have been identified. Mouse anti Human ZNF124 antibody, clone 4G4 is expected to bind to isoforms 1, 2 and 3. Binding to isoform 4 which lacks part of the immunogen sequeunce used to generate clone 4G4 has not been evaluated. Mouse anti Human ZNF124 antibody, clone 4G4 is not expected to react with isoform 5 which lacks the complete immunogen sequence used to generate this antibody (Uniprot : Q15937).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 121-230 of human ZNF124.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      transcription
      DNA binding
      nucleus
      zinc ion binding
      regulation of transcription, DNA-dependent
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional ZNF124 Antibody Formats

    Formats Clone Applications Sizes available
    ZNF124 Antibody : Preservative Free 4G4 WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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