ZAP 70 Antibody | ZAP-03

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  • Mouse anti Human ZAP 70
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    ZAP-03
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2513F *, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA2513
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • MCA2513 recognises human ZAP-70, a 70kD tyrosine protein kinase which associates with the T-cell receptor (TCR) zeta chain and undergoes phosphorylation following TCR stimulation. ZAP-70 is primarily expressed in T lymphocytes and natural killer cells, where it plays a key role in T-cell receptor (TCR) signalling. Recent studies suggest that ZAP-70 is also expressed in a population of normal immature B-cells and in B-cells from a sub-set of patients with chronic lymphocytic leukaemia.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Bacterially expressed fusion protein corresponding to the C-terminal region of human Zap-70.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      ATP binding
      cytosol
      intracellular protein kinase cascade
      non-membrane spanning protein tyrosine kinase activity
      immune response
      T cell receptor signaling pathway
      positive thymic T cell selection
      T cell receptor complex
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
      Flow Cytometry(1)
      (1)
      Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA2513 detects a band of approximately 70kDa.
    • Instructions For Use

    Additional ZAP 70 Antibody Formats

    Formats Clone Applications Sizes available
    ZAP 70 Antibody : Purified ZAP-03 F *, WB 0.2 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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