ZAP 70 Antibody | ZAP-03

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  • Mouse anti Human ZAP 70
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2513F*, WBdatasheet pdfdatasheet pdf0.2 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human ZAP-70 antibody, clone ZAP-03 recognizes human Tyrosine-protein kinase ZAP-70, also known as ZAP-70, 70 kDa zeta-chain associated protein or Syk-related tyrosine kinase. ZAP-70 is a 619 amino acid ~70 kDa tyrosine protein kinase which associates with the T-cell receptor (TCR) zeta chain and undergoes phosphorylation following TCR stimulation. ZAP-70 is primarily expressed in T lymphocytes and natural killer cells, where it plays a key role in T-cell receptor (TCR) signalling. ZAP-70 is also expressed in a population of normal immature B-cells, during B-cell development (Schweighoffer et al. 2003) and in B-cells from a sub-set of patients with chronic lymphocytic leukaemia (Crespo et al. 2003).

      Mutations in the ZAP70 gene can lead to the development of auto and immunodeficiency conditions, including Immunodeficiency 48 (IMD48) characterized by a selective absence of CD8 expressing T cells in the peripheral circulation despite their presence in the thymic cortex (Roifman et al. 1989) and Autoimmune disease, multisystem, infantile-onset, 2 (ADMIO2), a autosomal recessive condition presenting with skin blistering, inflammatory colitis, hypothyroidism, proteinuria and nephrotic syndrome (Chan et al. 2016).
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Bacterially expressed fusion protein corresponding to the C-terminal region of human Zap-70.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      ATP binding
      intracellular protein kinase cascade
      non-membrane spanning protein tyrosine kinase activity
      immune response
      T cell receptor signaling pathway
      positive thymic T cell selection
      T cell receptor complex
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
      Flow Cytometry(1)
      Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA2513 detects a band of approximately 70kDa.
    • Instructions For Use

    Additional ZAP 70 Antibody Formats

    Formats Clone Applications Sizes available
    ZAP 70 Antibody : Purified ZAP-03 F*, WB 0.2 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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