YES1 Antibody | 2D5

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YES1 Antibody | 2D5 gallery image 1

Western Blot analysis of YES1 expression in A-431 using Mouse anti Human YES1 (MCA5452Z)

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YES1 Antibody | 2D5 gallery image 2

Staining of paraffin-embedded human smooth muscle with Mouse anti Human YES1 (MCA5452Z)

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  • YES1 Antibody | 2D5 thumbnail image 1
  • YES1 Antibody | 2D5 thumbnail image 2
  • Mouse anti Human YES1:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    2D5
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5452ZP, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA5452Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human YES1 antibody, clone 2D5 recognizes human tyrosine-protein kinase Yes, also known as YES1, proto-oncogene c-Yes or p61-yes. YES1 is a 543 amino acid ~61 kDa non-receptor tyrosine kinase involved in a multiplicity of cellular processes. Yes1 is expressed in epithelial cells of renal proximal tubule and stomach as well as hematopoietic cells in the foetus. In adults it is expressed in the renal proximal tubules and some keratinocytes.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 1-544 of human YES1
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      protein binding
      T cell costimulation
      cytosol
      non-membrane spanning protein tyrosine kinase activity
      leukocyte migration
      ATP binding
      plasma membrane
      regulation of vascular permeability
      blood coagulation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin0.110 ug/ml
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional YES1 Antibody Formats

    Formats Clone Applications Sizes available
    YES1 Antibody : Preservative Free 2D5 P, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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