XPA Antibody

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  • Rabbit anti Human XPA
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP452P*, WBdatasheet pdfdatasheet pdf0.1 ml
    AHP452
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human XPA antibody recognizes the human DNA repair associated protein designated XPA (Xeroderma pigmentosum A).XPA is a zinc finger protein of ~31 kDa, although it demonstrates an electophoretic mobility of a~40-42 kDa doublet. XPA is involved in the nucleotide excision repair process.

      Rabbit anti Human XPA antibody recognises both normal and recombinant XPA, and shows no cross reactivity with other XP proteins.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Serum - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human XPA were raised by repeated immunisation of rabbits with highly purified antigen.
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Recombinant human XPA.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC for one month or at -20oC for longer.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      damaged DNA binding
      nucleotide-excision repair, DNA damage removal
      protein homodimerization activity
      metal ion binding
      protein domain specific binding
      nucleoplasm
      nucleotide binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/5000
      Immunohistology - Paraffin(1)1/50
      (1)
      This product requires heat mediated treatment of paraffin sections. Bio-Rad recommend the use of antigen unmasking fluid for this purpose. A two hour incubation with AHP452 is recommended.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional XPA Antibody Formats

    Formats Applications Sizes available
    XPA Antibody : Serum P*, WB 0.1 ml
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
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      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
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      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
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      Sheep anti Rabbit IgG:DyLight®680STAR36D680GA0.1 mgF, IF, WB
      STAR36D680GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Western Blotting

              References

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