XIAP Antibody

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Western blot analysis of Jurkat cell lysate probed with Rabbit anti XIAP antibody

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  • Rabbit anti XIAP
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2363WBdatasheet pdfdatasheet pdf50 µg
    AHP2363
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti XIAP recognizes XIAP (X-linked inhibitor of apoptosis protein) also known as IAP-3 (Inhibitor of apoptosis protein 3) or BIRC (baculoviral IAP repeat-containing protein 4). XIAP is an E3 ubiquitin ligase and acts as an endogenous inhibitor of cell death by suppressing caspases 3, 7, and 9 activities. The protein is also reported to play a role in the activation of the NF-kappa-B cell signaling pathway and the regulation of cyclins and cyclin-dependent kinase inhibitors (CDKIs). In addition to acting as an ubiquitin ligase XIAP has also been reported to have NEDD8 E3 ligase activity.
      XIAP has been identified as a potential tumor marker. In addition, aberrant XIAP regulation has been implicated in neurodegenerative and autoimmunity diseases. Mutations in XIAP have been shown to result in lymphoproliferative disorders.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MouseExpected from Sequence
      RatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      30% Glycerol
      0.5% Bovine Serum Albumin
      0.01% Thiomersal
    • Immunogen
      Synthetic peptide surrounding amino acid 230 of human XIAP
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.2 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      caspase inhibitor activity
      protein binding
      apoptosis
      response to DNA damage stimulus
      cytosol
      induction of apoptosis by intracellular signals
      anti-apoptosis
      zinc ion binding
      ligase activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.54.0 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti XIAP recognizes a band of approximately 57 kDa in Jurkat cell lysate
    • Instructions For Use

    Additional XIAP Antibody Formats

    Formats Applications Sizes available
    XIAP Antibody : Purified WB 50 µg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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