von Willebrand Factor Antibody

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von Willebrand Factor Antibody gallery image 1

Published customer image:
Sheep anti Human von Willebrand Factor antibody used for the evaluation of von Willebrand factor expression in isolated aortic cell preparations by immunofluorescence.
Image caption:
Characterization of the isolated cells.
(A) Representative phase contrast micrographs show the cell population collected at each of the four consecutive rounds of digestion (well 1 to 4) on the intimal (I) and adventitial (A) side of the aorta. In both cases, three main phenotypes were observed: small polygonal cells forming areas of cobblestone appearance, giant mono- or multinucleated cells and elongated cells. All three phenotypes were also detected at P1. Immunofluorescence analysis performed on each P0 population identified ECs by positive vWf staining (insert). The small polygonal mononucleated cells and the giant mono- or polynucleated morphologies, stained both for vWf. Rare elongated cells could also be vWf positive, their unusual shape likely being due to surrounding peer pressure. Magnification of the phase contrast pictures and immunofluorescence images is 10x and 63x, respectively. (B) Immunofluorescent micrographs showing the diversity of vWf presentation. vWf staining performed on adventitial aortic endothelial cells shows diverse and typical vWF staining patterns such as that of elongated WPBs throughout the cell body (d and e), residual vWf granules after degranulation of WPB or seen in cross section (a), reticular vWf around the nucleus corresponding to vWf re-synthesis after degranulation (b and c) or extracellular string of vWf (e). (C) Immunofluorescent micrographs of isolated cells double-stained for vWf together with either CD31 or VE-cadherin validates vWf staining. Small polygonal mono-nucleated cells and most of the giant mono- or polynucleated vWf positive cells also stained for CD31 or VE-cadherin at P0 and P2 and in IEC and AEC.

From: Leclercq A, Veillat V, Loriot S, Spuul P, Madonna F, Roques X, et al. (2015) A Methodology for Concomitant Isolation of Intimal and Adventitial Endothelial Cells from the Human Thoracic Aorta.
PLoS ONE 10(11): e0143144.

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von Willebrand Factor Antibody gallery image 2

Published customer image:
Sheep anti Human von Willebrand Factor antibody used for the evaluation of von Willebrand factor expression in isolated proliferating aortic cell preparations by immunofluorescence.
Image caption:
Analysis of proliferating cells: selection of EC-enriched fractions and validation of procedure.
(A) The left-hand morphologic diagram (M) shows the fraction used (arrow) for western blot analysis in (B) (WB). The high percentage of ECs was confirmed in the corresponding vWf immunofluorescent staining (IF), performed at P2 (200 cells were scored). (B) P2 ECs isolated with the recommended procedure from three patients: 8I, 1I (HD) and 10I and A, were lysed and analyzed for three EC and two mesenchymal markers by SDS PAGE. (C) Representative phase contrast (PC) images of the cells from the fractions selected for patient 10, stimulated or not by TGFbeta, before cell lysis and western blot analysis, are shown. The biochemical studies performed at P2 cross-validate the morphologic (performed at P0 and P1 stage by phase contrast imaging) and immunophenotypic (performed at P0 and P2 stage by immunofluorescent staining) characterization of the isolated cells.

From: Leclercq A, Veillat V, Loriot S, Spuul P, Madonna F, Roques X, et al. (2015) A Methodology for Concomitant Isolation of Intimal and Adventitial Endothelial Cells from the Human Thoracic Aorta.
PLoS ONE 10(11): e0143144.

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von Willebrand Factor Antibody gallery image 3

Published customer image:
Sheep anti Human von Willebrand Factor antibody, used for the identification of von Willebrand expressing endothlial cells in the spinal cord of dark agouti rats with experimental acute encephalomyelitis by immunofluorescence.
Image caption:
SLPI expression within the spinal cord is associated with ED1-expressing macrophages or activated microglia in the acute phase (score 3.5, A) and with GFAP-positive astrocytes (B) and NeuN expressing neuronal cells (C) especially during the relapsing phase (score 3.5); there was no association of SLPI with von-Willebrand-factor positive endothelial cells (D). Magnification: 100x.

From: Mueller AM, Pedré X, Stempfl T, Kleiter I, Couillard-Despres S, Aigner L, Giegerich G, Steinbrecher A. Novel role for SLPI in MOG-induced EAE revealed by spinal cord expression analysis.
J Neuroinflammation. 2008 May 26;5:20.

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  • Sheep anti Human von Willebrand Factor
  • Sheep anti Human von Willebrand Factor
  • Sheep anti Human von Willebrand Factor:FITC
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP062TC *, E, F, IFdatasheet pdfdatasheet pdf0.1 ml
    AHP062T
    AHP062C *, E, F, ID, IFdatasheet pdfdatasheet pdf1 ml
    AHP062
    AHP062FC, F *datasheet pdfdatasheet pdf1 ml
    AHP062F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Sheep anti Human von Willebrand Factor antibody recognizes Human von Willebrand factor, a glycoprotein synthesized in endothelial cells and megakaryocytes and circulating in the blood as a noncovalent complex by association with factor VIII.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
    • Reconstitution
    • Preparation
      Purified IgG was prepared from serum by ion exchange chromatography
      Purified IgG was prepared from serum by ion exchange chromatography
      Purified IgG prepared by ion exchange chromatography from serum.
    • Antiserum Preparation
      Antisera to von Willebrand factor were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from serum by ion exchange chromatography
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.01%Benzamidine
      0.1%EACA
      1mMEDTA
      0.09%Sodium Azide
      0.01%Benzamidine
      0.1%EACA
      1mMEDTA
      0.09%Sodium Azide
    • Immunogen
      Purified human von Willebrand factor prepared from citrated human plasma
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Glycine buffered saline
      Glycine buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      protease binding
      immunoglobulin binding
      endoplasmic reticulum
      Weibel-Palade body
      collagen binding
      blood coagulation, intrinsic pathway
      glycoprotein binding
      platelet alpha granule lumen
      platelet degranulation
      platelet activation
      protein homodimerization activity
      protein N-terminus binding
      integrin binding
      cell-substrate adhesion
      chaperone binding
      protein homooligomerization
      proteinaceous extracellular matrix
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry
      ImmunodiffusionNeat
      Immunofluorescence
      Immunohistology - Paraffin
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry
      ImmunodiffusionNeat
      Immunofluorescence
      Immunohistology - Paraffin
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/501/100
      Flow Cytometry(1)
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Tonsil
    • Histology Positive Control Tissue
      Tonsil
    • Histology Positive Control Tissue
      Tonsil
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional von Willebrand Factor Antibody Formats

    Formats Applications Sizes available
    von Willebrand Factor Antibody : FITC C, F * 1 ml
    von Willebrand Factor Antibody : Purified C *, E, F, ID, IF 0.1 ml | 1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
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          Recommended Positive Controls

            Histology Controls

              Tonsil
              Tonsil
              Tonsil

              References

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