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von Willebrand Factor antibody

Sheep anti Human von Willebrand Factor

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
von Willebrand Factor

Product Code Applications Pack Size List Price Your Price Qty
AHP062T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * E F IF 0.1 ml loader
List Price Your Price
loader
AHP062
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * E F ID IF 1 ml loader
List Price Your Price
loader

Sheep anti Human von Willebrand Factor antibody recognizes Human von Willebrand factor, a glycoprotein synthesized in endothelial cells and megakaryocytes and circulating in the blood as a noncovalent complex by association with factor VIII.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG was prepared from serum by ion exchange chromatography
Antiserum Preparation
Antisera to von Willebrand factor were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from serum by ion exchange chromatography
Buffer Solution
Glycine buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.1% EACA
0.01% Benzamidine
1 mM EDTA
Immunogen
Purified human von Willebrand factor prepared from citrated human plasma
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Immunodiffusion Neat
Immunofluorescence
Immunohistology - Frozen 1
Immunohistology - Paraffin
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Tonsil

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
List Price Your Price
loader
Description Rabbit anti Sheep IgG (H/L):Biotin

References for von Willebrand Factor antibody

  1. Tait, A.S. et al. (2001) Phenotype changes resulting in high-affinity binding of von Willebrand factor to recombinant glycoprotein Ib-IX: analysis of the platelet-type von Willebrand disease mutations.
    Blood. 98: 1812-8.
  2. Kriehuber, E. et al. (2001) Isolation and characterization of dermal lymphatic and blood endothelial cells reveal stable and functionally specialized cell lineages.
    J Exp Med. 194: 797-808.
  3. Lee, L. et al. (2002) Identification of synovium-specific homing peptides by in vivo phage display selection.
    Arthritis Rheum. 46: 2109-20.
  4. Blades, M.C. et al. (2002) Stromal cell-derived factor 1 (CXCL12) induces human cell migration into human lymph nodes transplanted into SCID mice.
    J Immunol. 168 (9): 4308-17.
  5. Blagoveshchenskaya, A.D. et al. (2002) Selective and signal-dependent recruitment of membrane proteins to secretory granules formed by heterologously expressed von Willebrand factor.
    Mol Biol Cell. 13:1582-93.
  6. Friis,T. et al. (2003) A quantitative ELISA-based co-culture angiogenesis and cell proliferation assay.
    APMIS. 111: 658-68.
  7. Butthep, P. et al. (2004) Endothelial injury and altered hemodynamics in thalassemia.
    Clin Hemorheol Microcirc. 31: 287-93.
  8. Holmén, C. et al. (2005) Heterogeneity of human nasal vascular and sinusoidal endothelial cells from the inferior turbinate.
    Am J Respir Cell Mol Biol. 32: 18-27.
  9. View The Latest Product References
  10. Zhu, B. et al. (2005) Cyclic GMP-specific phosphodiesterase 5 regulates growth and apoptosis in pulmonary endothelial cells.
    Am J Physiol Lung Cell Mol Physiol. 289: L196-206.
  11. Glinert, I.S. et al. (2008) Photodynamic ablation of a selected rat embryo: a model for the treatment of extrauterine pregnancy.
    Hum Reprod. 23: 1491-8.
  12. Mueller, A.M. et al. (2008) Novel role for SLPI in MOG-induced EAE revealed by spinal cord expression analysis.
    J Neuroinflammation. 5: 20.
  13. Renault, M.A. et al. (2009) The Hedgehog transcription factor Gli3 modulates angiogenesis.
    Circ Res. 105: 818-26.
  14. Babich, V. et al. (2009) Differential effect of extracellular acidosis on the release and dispersal of soluble and membrane proteins secreted from the Weibel-Palade body.
    J Biol Chem. 284: 12459-68.
  15. Kiskin, N.I. et al. (2010) Protein mobilities and P-selectin storage in Weibel-Palade bodies.
    J Cell Sci. 123: 2964-75.
  16. Chowdhury, F. et al. (2010) Interactions between endothelial cells and epithelial cells in a combined cell model of airway mucosa: effects on tight junction permeability.
    Exp Lung Res. 36: 1-11.
  17. Knipe, L. et al. (2010) A revised model for the secretion of tPA and cytokines from cultured endothelial cells.
    Blood. 116: 2183-91.
  18. Johnson, L.A. & Jackson, D.G. (2010) Inflammation-induced secretion of CCL21 in lymphatic endothelium is a key regulator of integrin-mediated dendritic cell transmigration.
    Int Immunol. 22 (10): 839-49.
  19. Johnson, L.A. and Jackson, D.G. (2010) Inflammation-induced secretion of CCL21 in lymphatic endothelium is a key regulator of integrin-mediated dendritic cell transmigration.
    Int Immunol. 22: 839-49.
  20. Johnson, L.A. & Jackson, D.G. (2013) The chemokine CX3CL1 promotes trafficking of dendritic cells through inflamed lymphatics.
    J Cell Sci. 126 (Pt 22): 5259-70.
  21. Leclercq, A. et al. (2015) A Methodology for Concomitant Isolation of Intimal and Adventitial Endothelial Cells from the Human Thoracic Aorta.
    PLoS One. 10 (11): e0143144.
  22. Cheung, K. et al. (2015) CD31 signals confer immune privilege to the vascular endothelium.
    Proc Natl Acad Sci U S A. 112 (43): E5815-24.
  23. Zhang, J. et al. (2017) Potential Antigens Involved in Delayed Xenograft Rejection in a Ggta1/Cmah Dko Pig-to-Monkey Model.
    Sci Rep. 7 (1): 10024.
  24. Rivera, J.R. & Czuprynski, C.J. (2019) Procoagulant activity of bovine neutrophils incubated with conditioned media or extracellular vesicles from Histophilus somni. stimulated bovine brain endothelial cells.
    Vet Immunol Immunopathol. 211: 49-57.
  25. Kuna, V.K. et al. (2019) Human fetal kidney cells regenerate acellular porcine kidneys via upregulation of key transcription factors involved in kidney development.
    AIMS Cell and Tissue Engineering. 3 (1): 26-46.
  26. Boeckelmann, D. et al. (2020) Panel Sequencing Identified a Novel Splice Site Mutation in Hermansky-Pudlak Syndrome Type 1 Patients
    Arch Clin Med Case Rep. 4 (6): 1172-81
  27. Lopes-da-Silva, M. et al. (2019) A GBF1-Dependent Mechanism for Environmentally Responsive Regulation of ER-Golgi Transport.
    Dev Cell. 49 (5): 786-801.e6.

ELISA

Flow Cytometry

Immunofluorescence

Synonyms
Factor VIII Related Antigen
RRID
AB_322241
UniProt
P04275
Entrez Gene
VWF
GO Terms
GO:0001948 glycoprotein binding
GO:0002020 protease binding
GO:0002576 platelet degranulation
GO:0051087 chaperone binding
GO:0005783 endoplasmic reticulum
GO:0005178 integrin binding
GO:0005518 collagen binding
GO:0005578 proteinaceous extracellular matrix
GO:0007597 blood coagulation, intrinsic pathway
GO:0019865 immunoglobulin binding
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0031589 cell-substrate adhesion
GO:0033093 Weibel-Palade body
GO:0042803 protein homodimerization activity
GO:0051260 protein homooligomerization
GO:0047485 protein N-terminus binding

AHP062T

150274 152990 155612

AHP062

149294 151885 152989 155037

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