Vitamin D Receptor Antibody | 2F4

Vitamin D Receptor Antibody | 2F4 gallery image 1

Staining of paraffin embedded human lung with Mouse anti Human Vitamin SD Receptor clone 2F4:Azide Free (MCA3543Z)

Vitamin D Receptor Antibody | 2F4 gallery image 2

Published customer image:
Mouse anti Human vitamin D receptor antibody, clone 2F4 used for the detection of vitamin D receptor expressing cells in breast cancer tissues by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative immunohistochemical figures of ductal infiltrating breast cancer with low VDR expression (score 3) (a) and with high VDR expression (score 8) (b). Scale bar 30 µ. (c) The MCA graph demonstrated that phenotype AA is located in the quadrant containing the most aggressive parameters (T3 tumours, HER2 positive, ER negative and G3 in contrast to phenotype GG which is associated to more favorable bio-pathological parameters [T1 tumours, N0, G1, ki67 (-)].

From: Pulito C, Terrenato I, Di Benedetto A, Korita E, Goeman F, et al. (2015) Cdx2 Polymorphism Affects the Activities of Vitamin D Receptor in Human Breast Cancer Cell Lines and Human Breast Carcinomas. PLoS ONE 10(4): e0124894.

Vitamin D Receptor Antibody | 2F4 gallery image 3

Published customer image:
Mouse anti Human vitamin D receptor antibody, clone 2F4 (MCA3543A) used to evaluate VDR expression in breast cancer tissue by immunohistochemistry.
Image caption:
VDR, RXR and PPARγ immunostaining in BRCA1 mutated breast cancer. VDR, RXR and PPARγ were detected by immunohistochemistry in BRCA1 mutated breast cancer. Representative images are shown; magnification: a-c: 10 × lens, d-f: 25 × lens

From:Heublein S, Mayr D, Meindl A, Kircher A, Jeschke U, Ditsch N.
Vitamin D receptor, Retinoid X receptor and peroxisome proliferator-activated receptor γ are overexpressed in BRCA1 mutated breast cancer and predict prognosis.
J Exp Clin Cancer Res. 2017 Apr 20;36(1):57.

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  • Mouse anti Human Vitamin D Receptor:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3543ZP*datasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human Vitamin D receptor antibody, clone 2F4 recognizes the human Vitamin D3 receptor, also known as 1,25-dihydroxyvitamin D3 receptor or Nuclear receptor subfamily 1 group I member 1. The vitamin D receptro is a 427 amino acid ~ 48kDa member of the nuclear hormone receptor family containing a single nuclear receptor DNA-binding domain
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 1 - 428 of human Vitamin D receptor.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      steroid hormone receptor activity
      vitamin D3 receptor activity
      positive regulation of vitamin D 24-hydroxylase activity
      regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
      retinoid X receptor binding
      negative regulation of cell proliferation
      zinc ion binding
      sequence-specific DNA binding transcription factor activity
      regulation of calcidiol 1-monooxygenase activity
      negative regulation of gene-specific transcription from RNA polymerase II promoter
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin(1)0.110 ug/ml
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Vitamin D Receptor Antibody Formats

    Formats Clone Applications Sizes available
    Vitamin D Receptor Antibody : Preservative Free 2F4 P* 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunohistology - Paraffin


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