Visfatin

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  • Recombinant Human Visfatin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP201FNdatasheet pdfdatasheet pdf25 µg
    PHP201
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Visfatin is a putative cytokine originally identified as PBEF (Pre-B cell-enhancing factor), subsequently determined to be a nicotinamide phosphoribosyltransferase (NAMPT) enzyme and member of the NAPRTase family, involved in catalyzing the biosynthesis of NAD from nicotinamide.

      Visfatin is highly expressed in bone marrow, muscle and liver tissue and increases in expression in response to endotoxin, in amniotic epithelial cells following lipopolysaccharide (LPS) stimulation, and during the activation of T and B cells, dendritic cells and macrophages, suggesting a role in inflammatory processes.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 0.25ml distilled water.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

      N.B. Due to solubility reasons, it is recommended that the solution be kept at low pH..
    • Preparation
      Purified recombinant visfatin expressed in E.coli.
    • Preservative Stabilisers
      None present
    • Activity
      The ED 50 was determined by the dose-dependant proliferation of the RPMI 8226 cells. The expected ED 50 for this effect is 15.0-20.0 ng/ml.
    • Purity
      >98% by SDS PAGE and HPLC analysis.
    • Approx. Protein Concentrations
      Total protein concentration 0.1mg/ml after reconstitution.
    • Molecular Weight
      52.5kD (465 amino acid residues).
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <1EU/ug
    • Test Principle
    • Buffer Solution
      10mM HCl
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      NAD biosynthetic process
      positive regulation of cell proliferation
      cytosol
      nicotinamide metabolic process
      positive regulation of nitric-oxide synthase biosynthetic process
      nicotinate-nucleotide diphosphorylase (carboxylating) activity
      cytokine activity
      cell-cell signaling
      signal transduction
      nicotinamide phosphoribosyltransferase activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Visfatin Formats

    Formats Applications Sizes available
    Visfatin : Rec. Protein FN 25 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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