Visfatin Antibody | 4D5

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Western blot analysis of human visfatin expression in the human embryonic kidney 293T cell line using Mouse anti Human visfatin (MCA4632GA)

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  • Mouse anti Human Visfatin
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  • Product Type
    Monoclonal Antibody
  • Clone
    4D5
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4632GAE, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA4632GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Visfatin, clone 4D5 recognizes human Visfatin, a putative cytokine originally identified as PBEF (Pre-B cell-enhancing factor), subsequently determined to be a nicotinamide phosphoribosyltransferase (NAMPT) enzyme and member of the NAPRTase family, involved in catalyzing the biosynthesis of NAD from nicotinamide.

      Visfatin is highly expressed in bone marrow, muscle and liver tissue and increases in expression in response to endotoxin, in amniotic epithelial cells following lipopolysaccharide (LPS) stimulation, and during the activation of T and B cells, dendritic cells and macrophages, suggesting a role in inflammatory processes.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G ascities
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3) 10% Glycerol
    • Immunogen
      Recombinant human visfatin (amino acids 1-491) purified from Escherichia coli.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse FO myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      NAD biosynthetic process
      positive regulation of cell proliferation
      nicotinamide metabolic process
      nicotinamide phosphoribosyltransferase activity
      cytosol
      signal transduction
      cytokine activity
      nicotinate-nucleotide diphosphorylase (carboxylating) activity
      positive regulation of nitric-oxide synthase biosynthetic process
      cell-cell signaling
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/10001/3000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA4632GA detects a band of approximately 56 kDa in 293T cell lysates.
    • Instructions For Use

    Additional Visfatin Antibody Formats

    Formats Clone Applications Sizes available
    Visfatin Antibody : Purified 4D5 E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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