VISA Antibody

VISA Antibody gallery image 1

Western blot analysis of rat brain lysate probed with Rabbit anti Human VISA (AHP1702) at 0.5(A), 1(B) and 2(C) ug/ml

VISA Antibody gallery image 2

Immunohistological staining of mouse brain with Rabbit anti Human VISA (AHP1702)

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  • Rabbit anti VISA
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1702P*, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti VISA antibody recognizes the human Mitochondrial antiviral-signaling protein (MAVS), also known as Virus-induced-signalling-adaptor (VISA), and KIAA1271, VISA is a 540 amino acid outer mitochondrial membrane protein containing a single CARD domain. VISA is an important protein required for innate immune defence against viruses (Huang et al. 2014).

      VISA acts downstream of DDX58 and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). VISA is believed to activate the same pathways following detection of extracellular dsRNA by TLR3 and it has been hypothosized that VISA is involved in the regulation of apoptosis following viral infection (Lei et al. 2009).

      VISA is a ubiquitously expressed protein present in a range of cell types including T-cells, monocytes and epithelial cells, with highest levels in tissues of the heart, skeletal muscle, liver, placenta and in peripheral blood leukocytes. Multiple isoforms of VISA generated by alternative splicing have been identified. Rabbit anti VISA antibody is reactive with full length VISA isoform 1. It does not react with isoforms 2,3,5 or 6, reactivity with isoform 4 is unknown.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human VISA were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
    • Immunogen
      A peptide corresponding to a 17 amino acid sequence from near the centre of Human VISA.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.51ug/ml
      Immunohistology - Paraffin(1)2.5ug/ml
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Mouse brain
    • Immunofluorescence
    • Western Blotting
      AHP1702 detects a band of approximately 56kDa in Rat brain tissue lysate.
    • Instructions For Use

    Additional VISA Antibody Formats

    Formats Applications Sizes available
    VISA Antibody : Purified P*, WB 0.1 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          Histar Detection SystemSTAR3000A50 TestsC, P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              Mouse brain

              Further Reading

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