VEGF Receptor 3 Antibody | 5B5

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VEGF Receptor 3 Antibody | 5B5 gallery image 1

Western Blot analysis of VEGF RECEPTOR 3 expression in MES-SA/Dx5 using Mouse anti Human VEGF RECEPTOR 3 (MCA5331Z)

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  • VEGF Receptor 3 Antibody | 5B5 thumbnail image 1
  • Mouse anti Human VEGF Receptor 3:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    5B5
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5331ZWBdatasheet pdfdatasheet pdf0.1 mg
    MCA5331Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human VEGF receptor 3 antibody, clone 5B5 recognizes human vascular endothelial growth factor receptor 3, also known as Fms-like tyrosine kinase 4, FLT-4 or Tyrosine-protein kinase receptor FLT4. FLT-4 is a 1339 amino acid ~160 kDa single pass type 1 transmembrane glycoprotein receptor for VEGFC and VEGFD, playing an essential role in adult lymphangiogenesis.

      Three isoforms produced by alternative splicing have been described. Mouse anti Human VEGF receptor 3 antibody, clone 5B5 binds to the canonical isoform 1, isoforms 2 and 3 also preserve the complete unaltered immunogen sequence used to generate clone 5B5 and therefore also be expected to be recognized by the antibody.

      Mutations of the FLT4 gene can lead to Lymphedema, hereditary, 1A (LMPH1A), a chronic condition with swelling of the extremities caused by alterations in lymph flow (Irrthum et al. 2000). Mutations can also lead to increases susceptibility to Hemangioma, capillary infantile (HCI), a condition characterised by sharply demarcated skin hemangiomas present at birth or within the first few months after birth as a result of highly proliferative capillary endothelial growth. These generally undergo regression and involution (Walter et al. 2002).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 34-133 of human VEGF receptor 3.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      protein phosphorylation
      integral to plasma membrane
      protein binding
      vascular endothelial growth factor receptor activity
      ATP binding
      positive regulation of cell proliferation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional VEGF Receptor 3 Antibody Formats

    Formats Clone Applications Sizes available
    VEGF Receptor 3 Antibody : Preservative Free 5B5 WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

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