Vasoactive Intestinal Peptide Antibody

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Vasoactive Intestinal Peptide Antibody gallery image 1

Formalin fixed, paraffin embedded human pancreas stained with Rabbit anti Human vasoactive intestinal peptide (9535-0204)

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Rabbit anti vasoactive intestinal peptide antibody used to identify VIP reactive cells in therumen of a pregnant goat by immunohistochemistry on formalin fixed paraffin embedded tissue sections.
Image caption:
Distribution of immunoreactivity in the goat rumen. (A) Synaptophysin (SPY)-positive staining in the tunica muscularis and myenteric plexus (arrow) in a section of rumen wall at 64 days (43" gestation). (B) Non-neuronal enolase (NNE) immunoreactivity in the epithelial layer (arrow), lamina propria-submucosa, tunica muscularis, and myenteric plexus (arrow) at 64 days (43% gestation). (C) Glial fibrillary acidic protein (GFAP)-positive staining at 64 days (43% gestation) in the lamina propria-submucosa, tunica muscularis, serosa, and myenteric plexus, (arrows) and within ruminal papillae. (D) Vimentin (VIM)-specific immunostaining in the mesenchymal cells and serosa of the ruminal wall at 39 days (25% gestation). (E) Neuropeptide Y (NPY)-positive staining in the lamina propria-submucosa, tunica muscularis, and myenteric plexus (arrows) at 113 days (75% gestation). (F) Vasoactive intestinal peptide (VIP)-specific staining at 113 days (75% gestation) in the lamina propria-submucosa. Intense immunostaining was observed in the tunica muscularis and myenteric plexus (arrows). Scale bars = 20 μm (D), 25 μm (A, B, E, and F), or 30 μm (C).

From: Garcia A, Masot J, Franco A, Gazquez A, Redondo E. Immunohistochemical evaluation of the goat forestomach during prenatal development.
J Vet Sci. 2014;15(1):35-43.

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Rabbit anti vasoactive intestinal peptide antibody used to identify VIP reactive cells in the reticulum of a pregnant goat by immunohistochemistry on formalin fixed paraffin embedded tissue sections.
Image caption:
Distribution of immunoreactivity in the goat reticulum. (A) SYN-positive staining in the lamina propria-submucosa, tunica muscularis, serosa, and myenteric plexus (arrow) at 64 days (43%#37; gestation). (B) NNE-specific immunoreactivity visible in the epithelium (arrow), lamina propria-submucosa, tunica muscularis, serosa, and myenteric plexus (arrow) at 69 days (46% gestation). (C) GFAP-positive staining in the lamina propria-submucosa, tunica muscularis, and myenteric plexus (arrow) at 64 days (43% gestation). (D) VIM-specific immunostaining in the pluripotential blastemic tissue layer and serosa at 39 days (25% gestation). (E) NPY-positive staining in the lamina propria-submucosa, tunica muscularis, and myenteric plexus (arrow) at 75 days (50% gestation). (F) VIP-specific staining in the lamina propria-submucosa, tunica muscularis, and myenteric plexus (arrows) at 75 days (50% gestation). Scale bars = 25 μm (A, C, D, and E), 20 μm (B), or 30 μm (F).

From: Garcia A, Masot J, Franco A, Gazquez A, Redondo E. Immunohistochemical evaluation of the goat forestomach during prenatal development.
J Vet Sci. 2014;15(1):35-43.

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Vasoactive Intestinal Peptide Antibody gallery image 4

Published customer image:
Rabbit anti vasoactive intestinal peptide antibody used to identify VIP reactive cells in the omasum of a pregnant goat by immunohistochemistry on formalin fixed paraffin embedded tissue sections.
Image caption:
Distribution of immunoreactivity in the goat omasum. (A) SYN-positive staining in the lamina propria-submucosa, tunica muscularis, and myenteric plexus in the omasal wall at 75 days (50% gestation). (B) NNE-specific immunoreactivity in the epithelial layer, lamina propria-submucosa, and tunica muscularis at 64 days (75% gestation). (C) GFAP-positive staining within the myenteric plexus (arrow), lamina propria-submucosa, tunica muscularis, and serosa at 69 days (46% gestation). (D) VIM-specific immunostaining in the pluripotential blastemic tissue layer and serosa at 39 days (25% gestation). (E) NPY-positive staining in the lamina propria-submucosa, tunica muscularis, and myenteric plexus. An immunostained surface was visible in the connective tissue of the omasal laminae at 95 days (63% gestation). (F) VIP-positive staining in the connective tissue and tunica muscularis of the omasal laminae at 113 days (75% gestation). Scale bar = 25 µm (A, C, and E) or 20 µm (B, D, and F).

From: Garcia A, Masot J, Franco A, Gazquez A, Redondo E. Immunohistochemical evaluation of the goat forestomach during prenatal development.
J Vet Sci. 2014;15(1):35-43.

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Vasoactive Intestinal Peptide Antibody gallery image 5

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Insert meta-description and applications
Image caption:
Hippocampus and habenula in ChAT-tauGFP mice. (A) Flat-projected confocal image displaying (green) ChAT-tauGFP cells in the hippocampus. Cells and layers are counterstained with (red) Neurotrace 640/660 and DAPI (blue). Representative examples of Neurotrace/DAPI-positive ChAT-tauGFP cells in (B–E) habenula and (F–I) CA3 SP. (J–M) Representative example of a Neurotrace/DAPI-negative ChAT-tauGFP structure in the CA3 SO layer. (J-M, inset) Magnified view suggesting an en passant bouton. Dotted boxes in (A) show the relationship to (B–M).

From: Yi F, Catudio-Garrett E, Gábriel R, Wilhelm M, Erdelyi F, Szabo G, Deisseroth K, Lawrence J. Hippocampal "cholinergic interneurons" visualized with the choline acetyltransferase promoter: anatomical distribution, intrinsic membrane properties, neurochemical characteristics, and capacity for cholinergic modulation.
Front Synaptic Neurosci. 2015 Mar 6;7:4.

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  • Rabbit anti Vasoactive Intestinal Peptide
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    9535-0204Pdatasheet pdfdatasheet pdf0.5 ml
    9535-0204
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Vasoactive Intestinal Peptide antibody recognizes human vasoactive intestinal peptide, a secreted peptide that causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      BovineExpected from Sequence
      DogExpected from Sequence
      HorseExpected from Sequence
      MouseExpected from Sequence
      PigExpected from Sequence
      Rhesus MonkeyExpected from Sequence
      RatExpected from Sequence
      SheepExpected from Sequence
      Goatyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide corresponding to full-length mature vasoactive intestinal peptide conjugated to Keyhole Limpet Haemocyanin.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      neuropeptide hormone activity
      body fluid secretion
      G-protein coupled receptor protein signaling pathway
      positive regulation of cell proliferation
      extracellular region
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human small bowel
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Vasoactive Intestinal Peptide Antibody Formats

    Formats Applications Sizes available
    Vasoactive Intestinal Peptide Antibody : Purified P 0.5 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Human small bowel

              References

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