Vapb Antibody (PrecisionAbTM Antibody) | 4D8

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Vapb Antibody (PrecisionAb<sup>TM</sup> Antibody) | 4D8 gallery image 1

Western blot analysis of whole cell lysates probed with VAPB antibody followed by detection with HRP conjugated Goat anti Mouse IgG antibody (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 13 second exposure. Arrow points to VAPB (molecular weight 27 kDa)

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  • Vapb Antibody with Control Lysate
  • Mouse anti Vapb
(Rated 0.0 out of 5 based on 0 customer reviews)
    Mouse anti-human VAPB antibody recognizes vesicle-associated membrane protein-associated protein B/C, also known as VAMP-associated 33 kDa protein. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00454WBdatasheet pdfdatasheet pdf100 µl
      VMA00454
      VMA00454KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00454KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human VAPB antibody recognizes VAPB, also known as VAMP-associated 33 kDa protein or vesicle-associated membrane protein-associated protein B/C.

        The protein encoded by the VAPB gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking (provided by RefSeq, Jul 2008).

        Mouse anti Human VAPB antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti VAPB detects a band of approximately 27 kDa in HeLa cell lysate
        Anti VAPB detects a band of approximately 27 kDa in HeLa cell lysate
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
        Purified IgG prepared by affinity chromatography on Protein G from ascites
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
      • Immunogen
        Recombinant protein corresponding to amino acids 2-223 of human VAPB produced in E. Coli
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HeLa lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        structural molecule activity
        protein homodimerization activity
        protein heterodimerization activity
        Golgi apparatus
        enzyme binding
        sphingolipid metabolic process
        integral to membrane
        endoplasmic reticulum unfolded protein response
        plasma membrane
        virus-host interaction
        positive regulation of viral genome replication
        beta-tubulin binding
        cell death
        endoplasmic reticulum membrane
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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