USP9X Antibody (PrecisionAbTM Antibody) | OTI1D7

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USP9X Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI1D7 gallery image 1

Western blot analysis of whole cell lysates probed with USP9X antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 13 second exposure. Arrow points to USP9X (molecular weight 270 kDa)

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  • USP9X Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI1D7 thumbnail image 1
  • USP9X Antibody with Control Lysate
  • Mouse anti USP9X
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    USP9X antibody recognizes the deubiquitinase ubiquitin-specific peptidase 9 X linked, also known as carboxyl-terminal hydrolase FAF-x, Drosophila fat facets related X-linked or deubiquitinating enzyme FAF-X. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00191WBdatasheet pdfdatasheet pdf100 µl
      VMA00191
      VMA00191KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00191KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human USP9X antibody recognizes USP9X, also known as Drosophila fat facets related, X-linked, deubiquitinating enzyme FAF-X, fat facets in mammals, fat facets protein related, X-linked, fat facets protein-related, X-linked, hFAM, probable ubiquitin carboxyl-terminal hydrolase FAF-X, ubiquitin specific protease 9, X chromosome (fat facets-like Drosophila), ubiquitin thioesterase FAF-X, ubiquitin thiolesterase FAF-X, ubiquitin-specific processing protease FAF-X, ubiquitin-specific protease 9, X chromosome and ubiquitin-specific-processing protease FAF-X.

        USP9X is a member of the peptidase C19 family and encodes a protein that is similar to ubiquitin-specific proteases. Though USP9X is located on the X chromosome, it escapes X-inactivation. Mutations in USP9X have been associated with Turner syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized (provided by RefSeq, Jul 2008).

        Mouse anti Human USP9X antibody detects a band of 270 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti USP9X detects a band of approximately 270 kDa in HEK293 cell lysates.
        Anti USP9X detects a band of approximately 270 kDa in HEK293 cell lysates.
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
        Mouse monoclonal antibody purified by affinity chromatography from ascites.
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
      • Immunogen
        Recombinant protein corresponding to amino acids 2246-2570 of human USP9X (NP_001034680) produced in E. coli
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HEK293 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        cysteine-type endopeptidase activity
        ubiquitin-dependent protein catabolic process
        female gamete generation
        co-SMAD binding
        BMP signaling pathway
        transforming growth factor beta receptor signaling pathway
        cytoplasm
        mitosis
        protein deubiquitination
        ubiquitin thiolesterase activity
        cell division
        chromosome segregation
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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