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USP9X antibody | OTI1D7

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Anti USP9X Antibody, clone OTI1D7 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti USP9X Antibody, clone OTI1D7 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti USP9X Antibody, clone OTI1D7 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with USP9X antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 13 second exposure. Arrow points to USP9X (molecular weight 270 kDa)
Anti USP9X Antibody, clone OTI1D7 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of USP9X from HEK293 whole cell lysate. The Input lane is 10% input; the USP9X-IP lane is the immunoprecipitation with the USP9X antibody (2.5 μg, VMA00191); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 μg, MCA928). Western blot analysis was performed using USP9X antibody (1/1,000, VMA00191) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to USP9X.

Mouse anti USP9X

Product Type
PrecisionAb Monoclonal
Clone
OTI1D7
Isotype
IgG1
Format
Purified
Specificity
USP9X

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Product Code Applications Pack Size List Price Your Price Qty
VMA00191
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 100 µl loader
List Price Your Price
loader

Mouse anti Human USP9X antibody recognizes USP9X, also known as Drosophila fat facets related, X-linked, deubiquitinating enzyme FAF-X, fat facets in mammals, fat facets protein related, X-linked, fat facets protein-related, X-linked, hFAM, probable ubiquitin carboxyl-terminal hydrolase FAF-X, ubiquitin specific protease 9, X chromosome (fat facets-like Drosophila), ubiquitin thioesterase FAF-X, ubiquitin thiolesterase FAF-X, ubiquitin-specific processing protease FAF-X, ubiquitin-specific protease 9, X chromosome and ubiquitin-specific-processing protease FAF-X.

USP9X is a member of the peptidase C19 family and encodes a protein that is similar to ubiquitin-specific proteases. Though USP9X is located on the X chromosome, it escapes X-inactivation. Mutations in USP9X have been associated with Turner syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized (provided by RefSeq, Jul 2008).

Mouse anti Human USP9X antibody detects a band of 270 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti USP9X detects a band of approximately 270 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein corresponding to amino acids 2246-2570 of human USP9X (NP_001034680) produced in E. coli
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q93008
Entrez Gene
USP9X
GO Terms
GO:0007059 chromosome segregation
GO:0004197 cysteine-type endopeptidase activity
GO:0004221 ubiquitin thiolesterase activity
GO:0005737 cytoplasm
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0007067 mitosis
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0007292 female gamete generation
GO:0016579 protein deubiquitination
GO:0030509 BMP signaling pathway
GO:0051301 cell division
GO:0070410 co-SMAD binding

VMA00191

240615

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