Western blotting analysis of HEK293 lysate (35μg protein in RIPA buffer) using Goat anti Human urokinase antibody (AHP1065) at 0.3μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.
Goat anti Human Urokinase antibody detects an epitope within the C-terminal of urokinase, a potent ~50 kDa serine protease also known as urokinase plasminogen activator (uPA). uPA cleaves and activates plasminogen to form plasmin and is involved in extracellular cellular matrix degradation and cell signalling. Recent studies have implicated uPA and its receptor in the pathogenesis of inflammation and immunity.
A specific polymorphism in the gene encoding uPA may be associated with late-onset Alzheimer's disease (Ertekin-Taner et al. 2005). The levels of uPA, uPAR and its soluble fragments have also been found to be increased in tissues, plasma and other body fluids of cancer patients and to be markers of cancer development and metastasis (Kim et al. 2016).
Purified IgG - liquid
Antisera to Urokinase were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Sodium Azide (NaN3)
Bovine Serum Albumin
Peptide with sequence C-HFLPWIRSHTKEEN from the C-terminal of the Urokinase sequence.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP1065 detects a band of approximately 48kDa in KEH 293 cell lysates.