Uncoupling Protein 4 Antibody

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  • Rabbit anti Human Uncoupling Protein 4
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    0100-0335E, WBdatasheet pdfdatasheet pdf1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Human Uncoupling Protein 4 antibody recognizes human mitochondrial uncoupling protein 4, also known as uncoupling Protein 4, UCP 4, Solute carrier family 25 member 27 or SLC25A27. Uncoupling protein 4 is a 323 amino acid, ~36kDa multiple pass, inner mitochondrial transmembrane protein bearing three Solcar repeats (UniProt: O95847)

      Uncoupling protein expression appears to be primarily associated with mitochondria of the central nervous syatem, with no protein being found in a number of peripheral organs when tested in immunohistochemistry, or western blotting in mice and rats. In mice uncoupling protein 4 appears in early to mid embryonic development coinciding with the initiation of neuronal differentiation and expression in mitochondria decreases with age, hinting at a possible role in neuronal differentiation (Smorodchenko et al. 2009). UCP4 facilitates the dissipation of heat across the inner mitochondrial membrane, by leaking protons across the membrane and uncoupling oxidative phosphorylation from ATP synthesis (Mao et al. 1999), the action of uncoupling protein 4 increasing proton concentration in the mitochondrial matrix may thus serve to increase ATP production by the class II complex (Ho et al. 2012). UCP4 is understood to be involved in thermoregulatory heat production and metabolism in brain.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide corresponding to the amino acids 271 - 283 of the native molecule (KSSTDCLIQA VQG) conjugated to Keyhole Limpet Haemocyanin.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 5.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      integral to membrane
      transmembrane transport
      mitochondrial inner membrane
      generation of precursor metabolites and energy
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      In reduced blots on bovine brown adipose tissue homogenates, this antibody recognises specific bands at ~34 & 44 kDa and other bands at ~50 & 60 kDa that can be blocked with KLH.
    • Instructions For Use

    Additional Uncoupling Protein 4 Antibody Formats

    Formats Applications Sizes available
    Uncoupling Protein 4 Antibody : Purified E, WB 1 ml
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*

          Recommended Positive Controls

            Histology Controls

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