Ubiquilin 2 Antibody | 5F5

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Ubiquilin 2 Antibody | 5F5 gallery image 1

Immunofluorescence showing UBIQUILIN 2 in A-431cells using Mouse anti Human UBIQUILIN 2:Azide Free (MCA3821Z)

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Ubiquilin 2 Antibody | 5F5 gallery image 2

Western blot analysis of UBQLN2 expression in A-431 using Mouse anti Human UBQLN2:Azide Free (MCA3821Z)

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  • Ubiquilin 2 Antibody | 5F5 thumbnail image 1
  • Ubiquilin 2 Antibody | 5F5 thumbnail image 2
  • Mouse anti Human Ubiquilin 2:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
    5F5
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3821ZIF, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA3821Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Ubiquilin 2 antibody, clone 5F5 recognizes human ubiquilin 2, also known as Chap1, DSK2 homolog or Protein linking IAP with cytoskeleton 2. Ubiquilin 2 is a 623 amino acid ~65 kDa protein with an important role in protein degradation pathways. It is highly expressed in cells during mitosis and at very low levels in non-mitotic cells.

      Mutations in the UBQLN2 gene are the cause of Amyotrophic lateral sclerosis 15, with or without frontotemporal dementia (ALS15), a neurodegenerative disease affecting neurons in the brain, brainstem and spinal cord eventually leading to fatal paralysis (Gilpin et al. 2015).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 555-625 of human ubiquilin 2
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Ubiquilin 2 Antibody Formats

    Formats Clone Applications Sizes available
    Ubiquilin 2 Antibody : Preservative Free 5F5 IF, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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