UBE2V2 Antibody (PrecisionAbTM Antibody)

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UBE2V2 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with UBE2V2 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 7 second exposure. Arrow points to UBE2V2 (molecular weight 16 kDa)

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  • UBE2V2 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • UBE2V2 Antibody with Control Lysate
  • UBE2V2 Antibody with Control Lysate
  • Rabbit anti UBE2V2
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  • Product Type
    PrecisionAb™ Polyclonal
  • Isotype
    Polyclonal IgG
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VPA00672WBdatasheet pdfdatasheet pdf100 µl
    VPA00672
    VPA00672KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
    VPA00672K
    VPA00672KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VPA00672KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
    -
    • Rabbit anti Human UBE2V2 antibody recognizes the ubiquitin-conjugating enzyme E2 variant 2, also known as 1 alpha,25-dihydroxyvitamin D3-inducible, DDVit 1, MMS2 homolog, enterocyte differentiation promoting factor or enterocyte differentiation-associated factor EDAF-1.

      Ubiquitin-conjugating enzyme E2 variant proteins constitute a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein encoded by UBE2V2 gene also shares homology with ubiquitin-conjugating enzyme E2 variant 1 and yeast MMS2 gene product. It may be involved in the differentiation of monocytes and enterocytes (provided by RefSeq, Jul 2008).

      Rabbit anti Human UBE2V2 antibody detects a band of 16 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
    • Target Species
      Human
    • Western Blotting
      Rabbit anti UBE2V2 detects a band of approximately 16 kDa in Jurkat cell lysates
      Rabbit anti UBE2V2 detects a band of approximately 16 kDa in Jurkat cell lysates
      Rabbit anti UBE2V2 detects a band of approximately 16 kDa in Jurkat cell lysates
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      Pack Size: 10 Westerns100μl Rabbit polyclonal antibody purified by affinity chromatography
      Pack Size: 2 Westerns20μl Rabbit polyclonal antibody purified by affinity chromatography
      Pack Size: 10 Westerns100μl Rabbit polyclonal antibody purified by affinity chromatography
      Pack Size: 2 Westerns20μl Rabbit polyclonal antibody purified by affinity chromatography
      Rabbit polyclonal antibody purified by affinity chromatography
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide
      2% Sucrose
      0.09% Sodium Azide
      2% Sucrose
      0.09% Sodium Azide
      2% Sucrose
    • Immunogen
      Synthetic peptide encompassing part of the N terminal region of human UBE2V2
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg Jurkat lysate lyophilized in RIPA buffer.
      400μg Jurkat lysate lyophilized in RIPA buffer.
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
    • Shelf Life
      As supplied, 12 months from date of despatch
      As supplied, 12 months from date of despatch
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories
      PrecisionAb™ is a trademark of Bio-Rad Laboratories
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      post-translational protein modification
      DNA double-strand break processing
      regulation of protein metabolic process
      nucleus
      protein binding
      regulation of DNA repair
      protein polyubiquitination
      small conjugating protein ligase activity
      UBC13-MMS2 complex
      cytoplasm
      cell proliferation
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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