Tubulin Gamma 2 Chain Antibody | 4F6

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Tubulin Gamma 2 Chain Antibody | 4F6 gallery image 1

Western blot analysis of TUBULIN GAMMA 2 CHAIN expression in HeLa using Mouse anti Human TUBULIN GAMMA 2 CHAIN:Azide Free (MCA4403Z)

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Tubulin Gamma 2 Chain Antibody | 4F6 gallery image 2

Immunofluorescence showing TUBULIN GAMMA 2 CHAIN in HeLa cells using Mouse anti Human TUBULIN GAMMA 2 CHAIN:Azide Free (MCA4403Z)

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  • Tubulin Gamma 2 Chain Antibody | 4F6 thumbnail image 1
  • Tubulin Gamma 2 Chain Antibody | 4F6 thumbnail image 2
  • Mouse anti Human Tubulin Gamma 2 Chain:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
    4F6
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4403ZIF*, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA4403Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Tubulin Gamma 2 chain antibody, clone 4F6 recognizes human tubulin gamma 2 chain, also known as TUBG2. Tubulin is the major constituent of microtubules. Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC, is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. By binding to the beta tubulin subunit of the tubulin molecule, it establishes the polarity of a microtubule leaving the alpha tubulin subunit exposed at the positive end.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein with a GST tag corresponding to amino acids 1 - 101 of human TUBG2.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      microtubule-based process
      cytosol
      GTPase activity
      G2/M transition of mitotic cell cycle
      structural molecule activity
      protein polymerization
      GTP binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.110 ug/ml
      Immunofluorescence(1)0.110 ug/ml
      (1)
      This product requires fixation of cells for 15 minutes. 4 % paraformaldehyde solution (NaOH in PBS) pH 7.4 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA4403Z detects a band of approximately 50 kDa in HeLa cell lysates
    • Instructions For Use

    Additional Tubulin Gamma 2 Chain Antibody Formats

    Formats Clone Applications Sizes available
    Tubulin Gamma 2 Chain Antibody : Preservative Free 4F6 IF*, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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