Tubulin Beta Chain Antibody | 3B3

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Tubulin Beta Chain Antibody | 3B3 gallery image 1

Western blot analysis of tubulin beta chain expression in Y-79 human retinoblastoma cell lysate using Mouse anti Human tubulin beta chain:Azide Free (MCA4593Z)

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Tubulin Beta Chain Antibody | 3B3 gallery image 2

Western blot analysis of NIH-3T3 murine embryonic fibroblast whole cell lysate probed with Mouse anti Human Tubulin beta antibody (MCA4593Z) followed by HRP conjugated Goat anti Mouse IgG, visualized using chemiluminescence

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  • Tubulin Beta Chain Antibody | 3B3 thumbnail image 1
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  • Mouse anti Human Tubulin Beta Chain:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    3B3
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4593ZWBdatasheet pdfdatasheet pdf0.1 mg
    MCA4593Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human tubulin beta chain antibody, clone 3B3 recognizes human tubulin beta chain, also known as TUBB or Tubulin beta-5 chain. Tubulin beta chain is a 444 amino acid ~50 kDa cytoskeletal protein.

      The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules.To form microtubules, the dimers of α- and β-tubulin bind to GTP and assemble onto the ends of microtubules while in the GTP-bound state. After being incorporated into the microtubule, the bound molecule of GTP will hydrolyse into GDP. TUBB is a subtype of human β-tubulin and is ubiquitously expressed with the highest levels present in spleen, thymus and immature brain.

      Mutations in the TUBB gene can lead to the development of Cortical dysplasia, complex, with other brain malformations 6 (CDCBM6), a condition of defective neuronal migration ond axonal guidance characterized by microcephaly, ataxia, and severe delayed psychomotor development (Breuss et al. 2012).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein with a GST tag corresponding to amino acids 1-445 of human TUBB
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      GTP binding
      MHC class I protein binding
      microtubule
      GTPase activity
      natural killer cell mediated cytotoxicity
      cytosol
      protein polymerization
      microtubule-based movement
      cellular component movement
      G2/M transition of mitotic cell cycle
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA4593Z detects a band of approximately 48 kDa in Y-79 cell lysates
    • Instructions For Use

    Additional Tubulin Beta Chain Antibody Formats

    Formats Clone Applications Sizes available
    Tubulin Beta Chain Antibody : Preservative Free 3B3 WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
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      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2a:FITCHCA037F0.1 mgF
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      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
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      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2a:HRPHCA037P0.1 mgE
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      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
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      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
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      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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