Tubulin Beta 2C Antibody | 1G3

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Tubulin Beta 2C Antibody | 1G3 gallery image 1

Western Blot analysis of tubulin beta 2C expression in HeLa using Mouse anti Human tubulin beta 2C (MCA4845Z)

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Tubulin Beta 2C Antibody | 1G3 gallery image 2

Immunofluorescence showing tubulin beta 2Cin HeLa cells using Mouse anti Human tubulin beta 2C (MCA4845Z)

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Tubulin Beta 2C Antibody | 1G3 gallery image 3

Western blot analysis of HEK293 cell lysate probed with Mouse anti Human tubulin beta 2C antibody (MCA4845Z) followed by HRP conjugated Goat anti Mouse IgG, visualized using chemiluminescence

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Tubulin Beta 2C Antibody | 1G3 gallery image 4

HeLA cells were fixed with 3% PFA and permeabilized with 0.3% Triton X-100. Nuclei were stained with 1.5 µg/ml DAPI. Immunofluorescence was carried out using MCA4845Z, a Mouse monoclonal antibody specific for Human tubulin beta 2C at 1/50. STAR117D488GA, Goat anti-Mouse IgG DyLight®488 was then applied at 1/50. Image was visualized on the Bio-Rad ZOE Fluorescent Cell Imager at 60x magnification.

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  • Mouse anti Human Tubulin Beta 2C:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    1G3
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4845ZIF, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA4845Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human tubulin beta 2C antibody, clone 1G3 recognizes human tubulin beta-4B chain, also known as tubulin beta 2 or tubulin beta 2C encoded by the TUBB4B gene. Tubulin beta 2C is a 445 amino acid ~50 kDa cytoskeletal protein, the predominant component of microtubules where it forms heterodimers with tubulin alpha chain components.

      Tubulin beta 2C is one of several tubulin beta isotypes incorporated into microtubules differentially according to cell specific requirements and interactions with other cytoskeletal components (Falconer et al. 1992). Tubulin beta 2C is up-regulated in sentinel lymph nodes from patients with colorectal cancer (He et al 2010).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Tubulin Beta 2C Antibody Formats

    Formats Clone Applications Sizes available
    Tubulin Beta 2C Antibody : Preservative Free 1G3 IF, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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