Tubulin Beta 2A Chain Antibody | 2B2

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Tubulin Beta 2A Chain Antibody | 2B2 gallery image 1

Western blot analysis of tubulin beta 2A chain expression in Jurkat human T cell leukemic cell lysate using Mouse anti Human tubulin beta 2A chain:Azide Free (MCA4184Z)

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Tubulin Beta 2A Chain Antibody | 2B2 gallery image 2

Western blot analysis of NIH 3T3 murine embryonic fibroblast whole cell lysate probed with Mouse anti Human tubulin beta 2a antibody (MCA4148Z) followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence

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  • Tubulin Beta 2A Chain Antibody | 2B2 thumbnail image 1
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  • Mouse anti Human Tubulin Beta 2A Chain:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    2B2
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4184ZWBdatasheet pdfdatasheet pdf0.1 mg
    MCA4184Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Tubulin beta 2A Chain antibody, clone 2B2 recognizes human tubulin beta 2A chain, also known as TUBB2A or Tubulin beta class IIa. Tubulin beta 2A chain is a 445 amino acid ~48 kDa cytoskeletal protein subtype of human β tubulin.

      The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules.To form microtubules, the dimers of α- and β-tubulin bind to GTP and assemble onto the ends of microtubules while in the GTP-bound state. After being incorporated into the microtubule, the bound molecule of GTP will hydrolyse into GDP.

      Mutations in the TUBB2A gene can lead to the development of Cortical dysplasia, complex, with other brain malformations 5 (CDCBM5), a condition characterized by altered neuronal migration and axonal guidance (Cushion et al. 2014).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein with a GST tag corresponding to amino acids 1-446 of human TUBB2A
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      microtubule
      protein binding
      protein polymerization
      microtubule-based movement
      GTP binding
      GTPase activity
      'de novo' posttranslational protein folding
      cytoplasm
      structural molecule activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA4184Z detects a band of approximately 48 kDa in Jurkat cell lysates
    • Instructions For Use

    Additional Tubulin Beta 2A Chain Antibody Formats

    Formats Clone Applications Sizes available
    Tubulin Beta 2A Chain Antibody : Preservative Free 2B2 WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
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      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2a:FITCHCA037F0.1 mgF
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      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
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      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
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      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
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      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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