Tubulin Alpha 4A Chain Antibody | 2E11

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Tubulin Alpha 4A Chain Antibody | 2E11 gallery image 1

Staining of paraffin embedded human testis with Mouse anti Human tubulin alpha 4A chain:Preservative Free (MCA3518Z)

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Tubulin Alpha 4A Chain Antibody | 2E11 gallery image 2

Immunofluorescence showing tubulin allpha 4A chain in HeLa cells using Mouse anti Human tubulin alpha 4A chain:Preservative Free (MCA3518Z)

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Western blot analysis of NIH 3T3 murine embryonic fibroblast whole cell lysate with Mouse anti Human tubulin alpha 4a chain (MCA3518Z) followed by HRP conjugated Goat anti Mouse IgG, visualized using chemiluminescence

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HeLA cells were fixed with 3% PFA and permeabilized with 0.3% Triton X-100. Nuclei were stained with 1.5 µg/ml DAPI. Immunofluorescence was carried out using MCA3518Z, a Mouse monoclonal antibody specific for the Human tubulin alpha 4A chain at 1/50. STAR117D488GA, Goat anti-Mouse IgG DyLight®488 was then applied at 1/50. Image was visualized on the Bio-Rad ZOE Fluorescent Cell Imager at 60x magnification.

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  • Mouse anti Human Tubulin Alpha 4A Chain:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
    2E11
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3518ZIF*, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA3518Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human Tubulin Alpha 4A chain antibody, clone 2E11 recognizes human tubulin alpha 4A chain, also known as TUBA4A or TUBA1
      The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules.To form microtubules, the dimers of α- and β-tubulin bind to GTP and assemble onto the ends of microtubules while in the GTP-bound state. After being incorporated into the microtubule, the bound molecule of GTP will hydrolyse into GDP.

      TUBA4A is a subtype of human a-tubulin.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
      Immunofluorescence(1)0.110 ug/ml
      Immunohistology - Paraffin(2)0.110 ug/ml
      (1)
      This product requires fixation of cells for 15 minutes. 4 % paraformaldehyde solution (NaOH in PBS) pH 7.4 is recommended for this purpose.
      (2)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA3518Z has been tested on a range of cell lines including HeLa and Jurkat where a band of approximately 50 kDa is detected.
    • Instructions For Use

    Additional Tubulin Alpha 4A Chain Antibody Formats

    Formats Clone Applications Sizes available
    Tubulin Alpha 4A Chain Antibody : Preservative Free 2E11 IF*, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2b:FITCHCA038F0.1 mgF
      HCA038F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2b:HRPHCA038P0.1 mgE
      HCA038P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      Human anti Mouse IgG2b:RPEHCA038PE100 TestsF
      HCA038PE

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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