TRIM25 Antibody (PrecisionAbTM Antibody)

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TRIM25 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with TRIM25 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 76 second exposure. Arrow points to TRIM25 (molecular weight 72 kDa)

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  • TRIM25 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Rabbit anti TRIM25
  • TRIM25 Antibody with Control Lysate
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  • Product Type
    PrecisionAb™ Polyclonal
  • Isotype
    Polyclonal IgG
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VPA00619WBdatasheet pdfdatasheet pdf100 µl
    VPA00619
    VPA00619KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VPA00619KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
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    • Rabbit anti Human TRIM25 antibody recognizes the E3 ubiquitin/ISG15 ligase TRIM25, also known as RING finger protein 147, estrogen-responsive finger protein, tripartite motif-containing protein 25, EFP or ubiquitin/ISG15-conjugating enzyme TRIM25.

      The protein encoded by TRIM25 gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene (provided by RefSeq, Jul 2008).

      Rabbit anti Human TRIM25 antibody detects a band of 72 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Target Species
      Human
    • Western Blotting
      Rabbit anti TRIM25 detects a band of approximately 72 kDa in HeLa cell lysates
      Rabbit anti TRIM25 detects a band of approximately 72 kDa in HeLa cell lysates
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      Rabbit polyclonal antibody purified by affinity chromatography
      20μl Rabbit polyclonal antibody purified by affinity chromatography
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide
      2% Sucrose
      0.09% Sodium Azide
      2% Sucrose
    • Immunogen
      Synthetic peptide encompassing part of the middle region of human TRIM25
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg HeLa lysate lyophilized in RIPA buffer.
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
    • Shelf Life
      As supplied, 12 months from date of despatch.
      As supplied, 12 months from date of despatch
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      PrecisionAb™ is a trademark of Bio-Rad Laboratories
    • Regulatory
      For research purposes only
    • GO Terms
      nucleus
      innate immune response
      negative regulation of type I interferon production
      cell junction
      ubiquitin-protein ligase activity
      response to virus
      interspecies interaction between organisms
      sequence-specific DNA binding transcription factor activity
      zinc ion binding
      cytosol
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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